Imperial/Wet Lab/Lab Notebook/2007-08-23
From 2007.igem.org
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#*Phosphoenolpyruvate to be added tomorrow | #*Phosphoenolpyruvate to be added tomorrow | ||
| - | Protocols can be found at [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/Notebook/General_Protocols#S30.2FS12_Cell_Extract#S30/S12 Cell Extract| S30/S12 Cell Extract] in the general protocols page | + | Protocols can be found at [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/Notebook/General_Protocols#S30.2FS12_Cell_Extract#S30/S12 Cell Extract | S30/S12 Cell Extract] in the general protocols page |
==Vesicles Formation with GFP== | ==Vesicles Formation with GFP== | ||
Revision as of 12:51, 26 October 2007
23 August 2007
Preparation of Reaction Buffer for S30 Cell Extract
- Calculated and weighed out required amounts of reagents
- Left mixture on the stirrer in the cold room overnight
- Phosphoenolpyruvate to be added tomorrow
Protocols can be found at [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/Notebook/General_Protocols#S30.2FS12_Cell_Extract#S30/S12 Cell Extract | S30/S12 Cell Extract] in the general protocols page
Vesicles Formation with GFP
Preparations
The desiccation and suspension stages of the vesicle preparation protocol were followed.
- One 100ml beaker was prepared with 0.5mg of powdered POPC in 50ml of dodecane.
- The suspension was sonicated for 30mins and left overnight in a 27°C incubator.
