Melbourne

Project overview

Background & references

Original Plan

Project This project aims to use light to form a solid fluorescent mass of E. coli where two light beams intersect in a suspension of cells. We've called our building system "Coliforming".

Motivation Possible applications in building complex scaffolds in tissue engineering.

Requirements The system requires six biological components.

1. Red photosensor
2. Blue photosensor
3. AND gate
4. GFP fluorescent reporter
5. Promotable surface expression of cadherins
6. Gas vesicle expression to produce neutrally bouyant bacteria

High Level System Design

• Induce or constitutively express gas vesicles for neutral bouyancy
• If Red & Blue light detected, then promote adhesion molecules.

Achievements

• Produced bouyant bacteria biobrick.
• Produced parts of other biological components required.

• Primary Reagents & disposables
• Protocols for secondary Reagents and waste disposal
• Protocols for standard methods
• Fixed Equipement & tools
• Software links

• Lab notebook/diary
• Parts Used by Melbourne
• Parts Created by Melbourne
• Meeting Minutes

• University of Melbourne
• Faculty of Biomedical Engineering

• Bio21 Institute
• Vice Chancellor (Research) Office