PennState/Project/Diauxie/DiauxieElimination
From 2007.igem.org
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| + | '''Test for Diauxie Elimination''' | ||
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| + | To test for diauxie elimination, constitutively expressed xylR transcriptional regulator is added following the promoter test constructs. | ||
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| + | <html><img src="http://openwetware.org/images/e/e6/Dtest.jpg" id="logo" name="logo" height="150" width="550" alt="" /></html> | ||
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| + | A possible model for cells naturally growing and cells growing with diauxie elimination is shown below. Elimination of diauxie with the use of CRP* could require too much of a time delay before reaching maximum growth. In this case the promoter region would need to be altered to resemble the consensus promoter sequence while keeping the xylR binding site to maintain xylose dependence. | ||
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| + | <img src="https://static.igem.org/mediawiki/2007/5/5a/Psuigem07diauxicgrowthcurves.jpg" id="logo" name="logo" height="300" width="300" alt="" /></a> | ||
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Revision as of 22:29, 26 October 2007
Test for Diauxie Elimination
To test for diauxie elimination, constitutively expressed xylR transcriptional regulator is added following the promoter test constructs.
A possible model for cells naturally growing and cells growing with diauxie elimination is shown below. Elimination of diauxie with the use of CRP* could require too much of a time delay before reaching maximum growth. In this case the promoter region would need to be altered to resemble the consensus promoter sequence while keeping the xylR binding site to maintain xylose dependence.
