PennState/Project/Dosimeter/InitialTest

From 2007.igem.org

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This construct uses the lambda operator to express GFP when induced by radiation.
This construct uses the lambda operator to express GFP when induced by radiation.
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|[[PennState/Project/Dosimeter|Dosimeter Introduction]]
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|[[PennState/Project/Dosimeter/InitialTest|Test Constructs]]
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|[[PennState/Project/Dosimeter/Dosimetry|Dosimetry]]
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|[[PennState/Project/Dosimeter/FinalConstructs|Final Constructs]]
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|}
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<h3><a rel="enclosure" href="https://2007.igem.org/PennState/Project/Diauxie" ="window.popup_player_258600 = window.open('https://2007.igem.org/PennState/Project/Dosimeter','post_258600','toolbar=no,scrollbars=no,directories=no,resizable=yes,width=360,height=305,top=20,left=20,location=no,menubar=no,status=yes,'); return false;">
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Introduction
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<span class="download">
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<a href="http://openwetware.org/images/9/97/Psuigem2007promoterimage.jpg">Image Link</a>
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</div>
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</div><div class="column">
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<div class="podcast">
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<h3><a rel="enclosure" href="https://2007.igem.org/PennState/Project/Dosimeter/InitialTest" ="window.popup_player_258600 = window.open('https://2007.igem.org/PennState/Project/Dosimeter/InitialTest','post_258600','toolbar=no,scrollbars=no,directories=no,resizable=yes,width=360,height=305,top=20,left=20,location=no,menubar=no,status=yes,'); return false;">
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Test Constructs
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</a></h3>
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<div class="screenshot">
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<!-- little white box thing --> <a rel="enclosure" href="https://2007.igem.org/PennState/Project/Dosimeter/InitialTest"
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<img src="http://openwetware.org/images/b/be/Psuigem2007RBSimage.jpg" width="133" height="100" alt="" />
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</a>
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<span class="download">
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<a href="http://openwetware.org/images/b/be/Psuigem2007RBSimage.jpg">Image Link</a>
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</span>
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</div>
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</div>
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</div>
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<div class="column"><div class="podcast">
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Dosimetry
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</a></h3>
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<div class="screenshot">
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<!-- little white box thing --> <a rel="enclosure" href="https://2007.igem.org/PennState/Project/Dosimeter/Dosimetry"
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<img src="http://openwetware.org/images/2/21/Psuigem2007geneimage.jpg" width="133" height="100" alt="" />
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</a>
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<span class="download">
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<a href="http://openwetware.org/images/2/21/Psuigem2007geneimage.jpg">Image Link</a>
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</span>
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</div>
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</div>
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</div>
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<div class="column"><div class="podcast">
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<!-- this is where you need to post the link to where it will go (the second one)-->
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<h3><a rel="enclosure" href="https://2007.igem.org/PennState/Project/Dosimeter/FinalConstructs" ="window.popup_player_258600 = window.open('https://2007.igem.org/PennState/Project/Dosimeter/FinalConstructs','post_258600','toolbar=no,scrollbars=no,directories=no,resizable=yes,width=360,height=305,top=20,left=20,location=no,menubar=no,status=yes,'); return false;">
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Final Constructs
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</a></h3>
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<div class="screenshot">
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<!-- little white box thing --> <a rel="enclosure" href="https://2007.igem.org/PennState/Project/Dosimeter/FinalConstructs"
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<img src="http://openwetware.org/images/9/9b/Psuigem2007TTimage.jpg" width="133" height="100" alt="" />
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</a>
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<span class="download">
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<a href="http://openwetware.org/images/9/9b/Psuigem2007TTimage.jpg">Image Link</a>
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</span>
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</div>
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</div>
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<div style="clear:both"></div>
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<!-- END OF LUC'S CODE -->
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</html>

Revision as of 22:42, 26 October 2007

Home Diauxie Project Dosimeter Project People Lab Parts Acknowledgements Links

Dosimeter Test Construct

To test the dosimeter, the assembled lambda region is placed in a plasmid preceding a ribosome binding site, the reporter green florescent protein, and a terminator. The promoter's sensitivity to radiation can be readily tested with flow cell cytometry and fluoresence plate reading



This construct uses the lambda operator to express GFP when induced by radiation.





Introduction

Image Link

Test Constructs

Image Link

Dosimetry

Image Link

Final Constructs

Image Link

Retrieved from "http://2007.igem.org/wiki/index.php/PennState/Project/Dosimeter/InitialTest"