PennState/Project/Dosimeter/Dosimetry
From 2007.igem.org
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| + | The nature of the bio-dosimeter means that it is an all or nothing response on a cellular level. While this makes the dosimeter easy to use and interpret, it increases the challeng to set it up as a practical way to monitor ionizing radiation. The threshold at which the dosimter triggers can be modified by testing various RBS sites in front of both Cl and Cro to change the rate of expression from the promoter. While changing the RBS strength will not have an affect on the maximum concentration of either protein as they are carefully regulated by the promoter, it will play a large role when the host's active RecA protease from the SOS response begins cleaving Cl. Here the replacement rate of the two proteins will determine whether the on or off state is the end result of irradiation. | ||
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Revision as of 23:24, 26 October 2007
The nature of the bio-dosimeter means that it is an all or nothing response on a cellular level. While this makes the dosimeter easy to use and interpret, it increases the challeng to set it up as a practical way to monitor ionizing radiation. The threshold at which the dosimter triggers can be modified by testing various RBS sites in front of both Cl and Cro to change the rate of expression from the promoter. While changing the RBS strength will not have an affect on the maximum concentration of either protein as they are carefully regulated by the promoter, it will play a large role when the host's active RecA protease from the SOS response begins cleaving Cl. Here the replacement rate of the two proteins will determine whether the on or off state is the end result of irradiation.
