Virginia/Projects/3
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| - | Butanol tolerance tests were performed by growing cells up in LB broth with different concentrations of butanol and then transferring the surviving cells from the highest butanol concentration to fresh LB with different butanol levels. This technique proved inadequate during the short time frame which we were able to perform experiments. Ideally, we would have grown the cells up between 500 and 1000 generations before increasing butanol concentration. Our results showed that e. coli typically grew well in the 0.8-1.2% (vol/vol) range. Occasionally, cells from higher butanol concentrations would survive, but would often be killed in the next experimental run. Therefore, above 1.2%, there was no consistent butanol tolerance. | + | Although the putative “butanol tolerance” gene was not propagated, butanol tolerance via directed evolution was attempted. Butanol tolerance tests were performed by growing cells up in LB broth with different concentrations of butanol and then transferring the surviving cells from the highest butanol concentration to fresh LB with different butanol levels. This technique proved inadequate during the short time frame which we were able to perform experiments. Ideally, we would have grown the cells up between 500 and 1000 generations before increasing butanol concentration. Our results showed that e. coli typically grew well in the 0.8-1.2% (vol/vol) range. Occasionally, cells from higher butanol concentrations would survive, but would often be killed in the next experimental run. Therefore, above 1.2%, there was no consistent butanol tolerance. |
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Revision as of 03:44, 27 October 2007
| HOME | PROJECT INTRO | APPROACH | PROCEDURES | RESULTS | [http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2007&group=Virginia BIOBRICKS] | [http://openwetware.org/wiki/IGEM:VGEM/2007/Notebook eNOTEBOOK] | [http://www.seas.virginia.edu/VGEM/ WEBSITE] |
Harvesting Cellulose and Light to Power Butanol Biosynthesis
Send comments to George McArthur
Results
Although the putative “butanol tolerance” gene was not propagated, butanol tolerance via directed evolution was attempted. Butanol tolerance tests were performed by growing cells up in LB broth with different concentrations of butanol and then transferring the surviving cells from the highest butanol concentration to fresh LB with different butanol levels. This technique proved inadequate during the short time frame which we were able to perform experiments. Ideally, we would have grown the cells up between 500 and 1000 generations before increasing butanol concentration. Our results showed that e. coli typically grew well in the 0.8-1.2% (vol/vol) range. Occasionally, cells from higher butanol concentrations would survive, but would often be killed in the next experimental run. Therefore, above 1.2%, there was no consistent butanol tolerance.
