Chiba/Sandbox/Home2
From 2007.igem.org
(→Making Affinity Tags) |
(→Making Affinity Tags) |
||
| Line 37: | Line 37: | ||
==Achievements Overview== | ==Achievements Overview== | ||
===Making Affinity Tags=== | ===Making Affinity Tags=== | ||
| + | [[Image:Chiba_flichisgene.png|frame|'''Fig.''' The short peptide with six histidine (“His-Tag”) was inserted into the fliC D3 domain.]] | ||
#Make the affinity tag by inserting the his-tag into the flagellar fillament. | #Make the affinity tag by inserting the his-tag into the flagellar fillament. | ||
#*[[Image:Chiba_check.png]] Sequence confirmed | #*[[Image:Chiba_check.png]] Sequence confirmed | ||
| Line 42: | Line 43: | ||
#*[[Image:Chiba_check.png]] Flagella strained with anti-flagella antibody | #*[[Image:Chiba_check.png]] Flagella strained with anti-flagella antibody | ||
#*[[Image:Chiba_check.png]] Phenotype confirmed | #*[[Image:Chiba_check.png]] Phenotype confirmed | ||
| - | #*[[Image:Chiba_check.png]] Affnity confirmed ( | + | #*[[Image:Chiba_check.png]] Affnity confirmed ([[Chiba/Engeneering_Flagella|more...]]]) |
| + | [[Image:Beads-Adsorption_Colony-Number.jpg|frame|'''Fig.'''Beads adsorption assay.]] | ||
===Making Communication Module=== | ===Making Communication Module=== | ||
Revision as of 03:35, 27 October 2007
|
Introduction | Project Design ( 1.Affinity Tag | 2.Communication Module | 3.Size Control ) | Making Marimos | Our Goal |
Chiba's Project
Our iGEM project is to make a Marimo-ish gathering of bacteria. Marimo is a green spherical shaped algae (shown in Fig.1), which is a popular living organism in Japan as a National Treasure.(<because of its shape?)
Motivation.
(more...)
Design
Our marimo bacteria requres as follows:
|
1.Affinity Tag. Make a His-tagged Flagella. We aimed to stick bacteria by displaying histidines (which bonds each other through metal ions) on the flagellar filament. |
|
2.Communication Module. Make a Bacterial Communication. We make 2 types of cell(sender&receiver) having different gene circuit. Senders sticking each other in advance send a signal to receivers and receivers grow affinity tags. |
|
3.Size Controler. Make an AHL localized region for quorum sensing. |
これを組み合わせて以下のように毬藻をつくる：
Achievements Overview
Making Affinity Tags
- Make the affinity tag by inserting the his-tag into the flagellar fillament.
Sequence confirmed
- Swarm confirmed
- Flagella strained with anti-flagella antibody
- Phenotype confirmed
- Affnity confirmed (more...])
Making Communication Module
- Making Senders
- 1.---
- 2.---
- Making Receivers
- 1.---
- 2.---
Controlling Size
- Improve Sender
- 前駆体合成酵素metKを入れてみた．＞結果？
- Improve Receiver
- LuxRの感度が良くなるmutation（論文より）を入れてみた．＞結果
- Localize AHL
- aiiAその１の回路（〜〜になるはず）＞結果
- aiiAその２の回路
- その３
