Chiba/Sandbox/Home2

From 2007.igem.org

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==Chiba's Project==
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==Project Overview==
Our iGEM project is to make a '''Marimo-ish gathering of bacteria'''. Marimo is a green spherical shaped algae (shown in Fig.1), which is a popular living organism in Japan as a National Treasure.(<because of its shape?)
Our iGEM project is to make a '''Marimo-ish gathering of bacteria'''. Marimo is a green spherical shaped algae (shown in Fig.1), which is a popular living organism in Japan as a National Treasure.(<because of its shape?)
Motivation.<br>
Motivation.<br>
(more...)
(more...)
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==Design==
 
Our marimo bacteria requres as follows:
Our marimo bacteria requres as follows:
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これを組み合わせて以下のように毬藻をつくる：
これを組み合わせて以下のように毬藻をつくる：
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===Methods===
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==Achievements Overview==
==Achievements Overview==
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===[[Chiba/Engeneering_Flagella|Making Affinity Tags]]===
===[[Chiba/Engeneering_Flagella|Making Affinity Tags]]===
[[Image:Chiba_flichisgene.png|frame|'''Fig.''' The short peptide with six histidine (“His-Tag”) was inserted into the fliC D3 domain.]]
[[Image:Chiba_flichisgene.png|frame|'''Fig.''' The short peptide with six histidine (“His-Tag”) was inserted into the fliC D3 domain.]]

Revision as of 03:50, 27 October 2007

Chiba logo.png

Introduction | Project Design ( 1.Affinity Tag | 2.Communication Module | 3.Size Control ) | Making Marimos | Our Goal
Acknowledgements | Team Members | [http://chem.tf.chiba-u.jp/igem/ iGEM Chiba Website] | メンバ連絡簿

Project Overview

Our iGEM project is to make a Marimo-ish gathering of bacteria. Marimo is a green spherical shaped algae (shown in Fig.1), which is a popular living organism in Japan as a National Treasure.(<because of its shape?) Motivation.
(more...)

Our marimo bacteria requres as follows:

Methods

Achievements Overview

Making Affinity Tags

Fig. The short peptide with six histidine (“His-Tag”) was inserted into the fliC D3 domain.
  1. Make the affinity tag by inserting the his-tag into the flagellar fillament.
    • Chiba check.png Sequence confirmed
    • Chiba check.png Swarm confirmed
    • Chiba check.png Flagella strained with anti-flagella antibody
    • Chiba check.png Phenotype confirmed
    • Chiba check.png Affnity confirmed

more...

Making Communication Module

  1. Making Senders
    • Chiba check.png AHL > GFP generator (constitutive aiiA) : BBa_T729006
    • Chiba check.png AHL > GFP & aiiA generator : BBa_I729005
    • Chiba check.png Sensitive AHL > GFP generator : BBa_I729004

[edit] Future Work inverter-aiiA


    • 1.---
    • 2.---
  2. Making Receivers
    • 1.---
    • 2.---

Controlling Size

  1. Improve Sender
    • 前駆体合成酵素metKを入れてみた．＞結果？
  2. Improve Receiver
    • LuxRの感度が良くなるmutation（論文より）を入れてみた．＞結果
  3. Localize AHL
    • aiiAその１の回路（〜〜になるはず）＞結果
    • aiiAその２の回路
    • その３

Making Marimo

Remaining Task(Work)

Our Goal

Future

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