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Revision as of 04:32, 27 October 2007 (view source) Silvapy (Talk | contribs) ← Older edit		Revision as of 04:38, 27 October 2007 (view source) Silvapy (Talk | contribs) Newer edit →
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	Primers		Primers
-	crtN-F   cloning of crtN  5’ccaaaAGATCTgtgaaacatacagcaaaaaacctgggt3’	+	crtN-F     3’ccaaaAGATCTgtgaaacatacagcaaaaaacctgggt5’
-	crtN-R   cloning of crtN  5’gctagCTCGAGttaGGATCCtcagtaactggctgacaagcct3’	+	crtN-R     5’gctagCTCGAGttaGGATCCtcagtaactggctgacaagcct3’
-	bch B|3'ttaGGATCCccgttcgccttggtttgacttact	+
		+	bch B       3'ttaGGATCCccgttcgccttggtttgacttact5'

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Revision as of 04:38, 27 October 2007

Biobricking of bch-gene cluster and crtN gene.

Gene:

bchB

bchE

bchI

bchL

bchM

bchN

[[crtNgene]]

crtN

PCR of crtN on Heliobacillus mobilis G/C content on annealing region

crtN-F 40% BglII CrtN-R 50% BamHI and XhoI

Primers

crtN-F 3’ccaaaAGATCTgtgaaacatacagcaaaaaacctgggt5’

crtN-R 5’gctagCTCGAGttaGGATCCtcagtaactggctgacaagcct3’

bch B 3'ttaGGATCCccgttcgccttggtttgacttact5'