From 2007.igem.org

Revision as of 04:38, 27 October 2007 (view source) Silvapy (Talk | contribs) ← Older edit		Revision as of 05:01, 27 October 2007 (view source) Silvapy (Talk | contribs) Newer edit →
Line 1:		Line 1:
-	Biobricking of bch-gene cluster and crtN gene.	+
		+	'''Materials'''
		+
		+	'''Methods'''
	Gene:		Gene:
Line 26:		Line 29:
	bch B      3'ttaGGATCCccgttcgccttggtttgacttact5'		bch B      3'ttaGGATCCccgttcgccttggtttgacttact5'
		+
		+	'''Results'''
		+
		+	'''Discussion'''Biobricking of bch-gene cluster and crtN gene.

---

Revision as of 05:01, 27 October 2007

Materials

Methods

Gene:

bchB

bchE

bchI

bchL

bchM

bchN

[[crtNgene]]

crtN

PCR of crtN on Heliobacillus mobilis G/C content on annealing region

crtN-F 40% BglII CrtN-R 50% BamHI and XhoI

Primers

crtN-F 3’ccaaaAGATCTgtgaaacatacagcaaaaaacctgggt5’

crtN-R 5’gctagCTCGAGttaGGATCCtcagtaactggctgacaagcct3’

bch B 3'ttaGGATCCccgttcgccttggtttgacttact5'

Results

DiscussionBiobricking of bch-gene cluster and crtN gene.