BerkiGEM2007-VaibhaviConstructionFile8
From 2007.igem.org
(Difference between revisions)
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<pre> | <pre> | ||
| - | Biobrickification of composite | + | Biobrickification of composite part: RBS (pBca 9145 – Bca1090) + katG, HPI gene |
--------------------------------------------------- | --------------------------------------------------- | ||
Digest pBca9145-Bca1145 (BglII/BsaI, 1783+970, L) | Digest pBca9145-Bca1145 (BglII/BsaI, 1783+970, L) | ||
Sub into pBca9145-Bca9205 (BamHI/BsaI/AlwNI, 1049+553+449, L) | Sub into pBca9145-Bca9205 (BamHI/BsaI/AlwNI, 1049+553+449, L) | ||
| - | Product is | + | Product is I716258 |
--------------------------------------------------- | --------------------------------------------------- | ||
No Oligos Needed | No Oligos Needed | ||
</pre> | </pre> | ||
Revision as of 20:31, 27 June 2007
Biobrickification of composite part: RBS (pBca 9145 – Bca1090) + katG, HPI gene --------------------------------------------------- Digest pBca9145-Bca1145 (BglII/BsaI, 1783+970, L) Sub into pBca9145-Bca9205 (BamHI/BsaI/AlwNI, 1049+553+449, L) Product is I716258 --------------------------------------------------- No Oligos Needed
