BerkiGEM2007-VaibhaviConstructionFile6
From 2007.igem.org
(Difference between revisions)
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Biobrickification of composite part: RBS (pBca 9145 – Bca1090) + AHSP gene | Biobrickification of composite part: RBS (pBca 9145 – Bca1090) + AHSP gene | ||
--------------------------------------------------- | --------------------------------------------------- | ||
| - | Digest | + | Digest pBca 9145 – Bca1090 (AlwNI/BamHI, 1538 + 553, L) |
| - | Sub into | + | Sub into I716055 (AlwNI/BglII, 1519 + 868, S) |
Product is I716256 | Product is I716256 | ||
--------------------------------------------------- | --------------------------------------------------- | ||
No Oligos Needed | No Oligos Needed | ||
</pre> | </pre> | ||
Latest revision as of 20:49, 27 June 2007
Biobrickification of composite part: RBS (pBca 9145 – Bca1090) + AHSP gene --------------------------------------------------- Digest pBca 9145 – Bca1090 (AlwNI/BamHI, 1538 + 553, L) Sub into I716055 (AlwNI/BglII, 1519 + 868, S) Product is I716256 --------------------------------------------------- No Oligos Needed
