Melb:Primary

From 2007.igem.org

(Difference between revisions)
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==Primary Reagents==
==Primary Reagents==
===Kits===
===Kits===
-
Miniprep kit  
+
*Miniprep kit  
-
Gel clean-up system
+
*Gel clean-up system
===Enzymes===
===Enzymes===
-
Restriction enzymes (in particular, NotI, EcoRI, XbaI, HindIII)
+
*Restriction enzymes (in particular, NotI, EcoRI, XbaI, HindIII)
-
Ligase
+
*Ligase
-
Competent cells (both for plasmid purification and protein expression)
+
*Competent cells (both for plasmid purification and protein expression)
-
DNA standard
+
*DNA standard
===Chemicals===
===Chemicals===
-
Ingredients for media (LB, SOB)
+
*Ingredients for media (LB, SOB)
-
Glycerol
+
*Glycerol
-
Buffers (including TAE and TE)
+
*Buffers (including TAE and TE)
-
Loading dye for DNA gel
+
*Loading dye for DNA gel
==Disposables==
==Disposables==
===TUBES:===
===TUBES:===
-
Falcon: 10ml, 50ml
+
*Falcon: 10ml, 50ml
-
microcentrifuge: 1.7ml
+
*microcentrifuge: 1.7ml
===Tips===
===Tips===
-
Tips (10, 200, 1000 ul)
+
*Tips (10, 200, 1000 ul)
===Other===
===Other===
-
Gloves (Large , Med, Small)
+
*Gloves (Large , Med, Small)
-
Scalpel
+
*Scalpels
-
Tube racks
+
*Tube racks
-
Freezer boxes
+
*Freezer boxes
-
Parafilm
+
*Parafilm

Revision as of 08:06, 1 July 2007

Contents

Primary Reagents & Disposables

Primary Reagents

Kits

  • Miniprep kit
  • Gel clean-up system

Enzymes

  • Restriction enzymes (in particular, NotI, EcoRI, XbaI, HindIII)
  • Ligase
  • Competent cells (both for plasmid purification and protein expression)
  • DNA standard

Chemicals

  • Ingredients for media (LB, SOB)
  • Glycerol
  • Buffers (including TAE and TE)
  • Loading dye for DNA gel

Disposables

TUBES:

  • Falcon: 10ml, 50ml
  • microcentrifuge: 1.7ml

Tips

  • Tips (10, 200, 1000 ul)

Other

  • Gloves (Large , Med, Small)
  • Scalpels
  • Tube racks
  • Freezer boxes
  • Parafilm