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Revision as of 22:05, 3 July 2007

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Contents 1 Primary Reagents 1.1 Kits 1.2 Enzymes 1.3 Chemicals 2 Disposables 2.1 TUBES: 2.2 Tips 2.3 Other

Primary Reagents

Kits

• Miniprep kit
• Gel clean-up system
• Site directed mutagenesis (stratagene)
  • Quickchange multi 200515(x10),200514(x30): 3-5 changes 1 primer each 50% <8Kb
  • Quickchange 200518(x30) 200519(x10): single change 2 primers 90% <8Kb
  • QuickchangeII XL 200522(x30) 200521(x10): single change 2 primers 80% >8Kb
• Primer design program : http://www.stratagene.com/sdmdesigner/default.aspx

Enzymes

• Restriction enzymes (in particular, NotI, EcoRI, XbaI, HindIII)
• Ligase
• Competent cells (both for plasmid purification and protein expression)
• DNA standard

Chemicals

• Ingredients for media (LB, SOB)
• Glycerol
• Buffers (including TAE and TE)
• Loading dye for DNA gel

Disposables

TUBES:

• Falcon: 10ml, 50ml
• microcentrifuge: 1.7ml
• PCR

Tips

• Tips (10, 200, 1000 ul)

Other

• Gloves (Large , Med, Small)
• Scalpels
• Tube racks
• Freezer boxes
• Parafilm