July 1
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* Dilutions of pL.LuxI.C made in M9 with IPTG conc 0,5,10,50,100,500,1000 uM | * Dilutions of pL.LuxI.C made in M9 with IPTG conc 0,5,10,50,100,500,1000 uM | ||
i) for FACS (in triplicate for a particular inoculum volume) | i) for FACS (in triplicate for a particular inoculum volume) | ||
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ii) for Microscopy (for 2 inoculum volumes: 0.1 & 0.01 uL/mL) | ii) for Microscopy (for 2 inoculum volumes: 0.1 & 0.01 uL/mL) | ||
* Dilutions of pL.LuxR.Y made in M9 with IPTG conc 0,5,10,50,100,500,1000 uM | * Dilutions of pL.LuxR.Y made in M9 with IPTG conc 0,5,10,50,100,500,1000 uM | ||
i) for FACS (in triplicate) | i) for FACS (in triplicate) | ||
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ii) for Microscopy (duplicate). | ii) for Microscopy (duplicate). | ||
Revision as of 12:27, 3 July 2007
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Experiments
- pL.LuxI.C was inoculated in 3 mL L/B and 3 uL 100 mg/mL Amp for equivalence expt to be done on July 2.
- pL.LuxR.Y was inoculated in 3 mL L/B and 3 uL 100 mg/mL Amp for equivalence expt to be done on July 2.
- Dilutions of pL.LuxI.C made in M9 with IPTG conc 0,5,10,50,100,500,1000 uM
i) for FACS (in triplicate for a particular inoculum volume)
ii) for Microscopy (for 2 inoculum volumes: 0.1 & 0.01 uL/mL)
- Dilutions of pL.LuxR.Y made in M9 with IPTG conc 0,5,10,50,100,500,1000 uM
i) for FACS (in triplicate)
ii) for Microscopy (duplicate).
