Wisconsin/Agarose Gel
From 2007.igem.org
(Difference between revisions)
(→1.2% Agarose Gel preperation) |
(→1.2% Agarose Gel preperation) |
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===1.2% Agarose Gel preperation=== | ===1.2% Agarose Gel preperation=== | ||
| - | 8 wells | + | *8 wells |
| - | 600mg Agarose | + | *600mg Agarose |
| - | 50mL TBE | + | *50mL TBE |
| - | 1uL 10mg/ml EtBr | + | *1uL 10mg/ml EtBr |
Mix agarose and TBE in flask, microwave to make sure agarose is dissolved in TBE. Add EtBr and pour into gel box. Make sure everything is submerged in TBE before adding DNA. | Mix agarose and TBE in flask, microwave to make sure agarose is dissolved in TBE. Add EtBr and pour into gel box. Make sure everything is submerged in TBE before adding DNA. | ||
Revision as of 18:34, 4 July 2007
1.2% Agarose Gel preperation
- 8 wells
- 600mg Agarose
- 50mL TBE
- 1uL 10mg/ml EtBr
Mix agarose and TBE in flask, microwave to make sure agarose is dissolved in TBE. Add EtBr and pour into gel box. Make sure everything is submerged in TBE before adding DNA.
