Wisconsin/Agarose Gel
From 2007.igem.org
(Difference between revisions)
(→1.2% Agarose Gel preperation) |
(→1.2% Agarose Gel preparation) |
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*1uL 10mg/ml EtBr | *1uL 10mg/ml EtBr | ||
| - | Mix agarose and TBE in flask, microwave to make sure agarose is dissolved in TBE. Add EtBr and pour into gel box. | + | Mix agarose and TBE in flask, microwave to make sure agarose is dissolved in TBE. Add EtBr and pour into gel box. |
| + | |||
| + | *2uL DNA | ||
| + | *12uL ddH<sub>2</sub>O | ||
| + | *4uL 5x Dye | ||
| + | *DNA Ladder | ||
| + | |||
| + | Mix everything in tube before adding to well and ake sure gel box is submerged in TBE before adding DNA. | ||
Revision as of 18:44, 4 July 2007
1.2% Agarose Gel preparation
- 8 wells
- 600mg Agarose
- 50mL TBE
- 1uL 10mg/ml EtBr
Mix agarose and TBE in flask, microwave to make sure agarose is dissolved in TBE. Add EtBr and pour into gel box.
- 2uL DNA
- 12uL ddH2O
- 4uL 5x Dye
- DNA Ladder
Mix everything in tube before adding to well and ake sure gel box is submerged in TBE before adding DNA.
