Wisconsin/Agarose Gel
From 2007.igem.org
(Difference between revisions)
(→1.2% Agarose Gel preparation) |
(→1.2% Agarose Gel preparation) |
||
| Line 6: | Line 6: | ||
Mix agarose and TBE in flask, microwave to make sure agarose is dissolved in TBE. Add EtBr and pour into gel box. | Mix agarose and TBE in flask, microwave to make sure agarose is dissolved in TBE. Add EtBr and pour into gel box. | ||
| + | |||
*2uL DNA | *2uL DNA | ||
Revision as of 19:24, 4 July 2007
1.2% Agarose Gel preparation
- 8 wells
- 600mg Agarose
- 50mL TBE
- 1uL 10mg/ml EtBr
Mix agarose and TBE in flask, microwave to make sure agarose is dissolved in TBE. Add EtBr and pour into gel box.
- 2uL DNA
- 12uL ddH2O
- 4uL 5x Dye
- DNA Ladder
Mix everything in tube before adding to well and ake sure gel box is submerged in TBE before adding DNA.
