Wisconsin/Agarose Gel
From 2007.igem.org
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(→1.2% Agarose Gel preparation) |
(→1.2% Agarose Gel preparation) |
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Mix agarose and TBE in flask, microwave to make sure agarose is dissolved in TBE. Add EtBr and pour into gel box. | Mix agarose and TBE in flask, microwave to make sure agarose is dissolved in TBE. Add EtBr and pour into gel box. | ||
| - | |||
*2uL DNA | *2uL DNA | ||
*12uL ddH<sub>2</sub>O | *12uL ddH<sub>2</sub>O | ||
| - | *4uL 5x Dye | + | *4uL 5x Loading Dye |
| - | *DNA Ladder | + | *4uL DNA Ladder for reference |
| - | Mix | + | Add water if DNA is less than 10uL and #uL of 1x loading dye = #uL total. Mix content in tube before adding it to well. Also make sure gel box is submerged in TAE/TBE before adding DNA. |
Revision as of 19:31, 4 July 2007
1.2% Agarose Gel preparation
- 8 wells
- 600mg Agarose
- 50mL TBE
- 1uL 10mg/ml EtBr
Mix agarose and TBE in flask, microwave to make sure agarose is dissolved in TBE. Add EtBr and pour into gel box.
- 2uL DNA
- 12uL ddH2O
- 4uL 5x Loading Dye
- 4uL DNA Ladder for reference
Add water if DNA is less than 10uL and #uL of 1x loading dye = #uL total. Mix content in tube before adding it to well. Also make sure gel box is submerged in TAE/TBE before adding DNA.
