July 6
From 2007.igem.org
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A scatter plot with each dot representing a cell was also plotted to see the variation of fluorescence with the change in aTc and IPTG concentrations. | A scatter plot with each dot representing a cell was also plotted to see the variation of fluorescence with the change in aTc and IPTG concentrations. | ||
| + | {|align="center" cellspacing="2" | ||
| + | |- | ||
| + | |*Red : 0 ng/ml aTc | ||
| + | |*Green : 1 ng/ml aTc | ||
| + | |- | ||
| + | |*Blue : 5 ng/ml aTc | ||
| + | |*Cyan : 10 ng/ml aTc | ||
| + | |- | ||
| + | |*Magenta : 20 ng/ml aTc | ||
| + | |*Yellow : 50 ng/ml aTc | ||
|} | |} | ||
Revision as of 05:37, 7 July 2007
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Experiments
Analysis
Today we compiled the data collected from all our open-loop experiments performed at various concentrations of aTc (ng/ml). The following matrix of information was obtained:
|
CFP mean values |
IPTG (uM) or luxR.YFP | |||||||
|---|---|---|---|---|---|---|---|---|
| 0 | 5 | 10 | 50 | 100 | 500 | 1000 | ||
|
aTc (ng/ml) or luxI.CFP | 0 | |||||||
| 1 | ||||||||
| 5 | ||||||||
| 10 | ||||||||
| 20 | ||||||||
| 50 | ||||||||
|
YFP mean values |
IPTG (uM) or luxR.YFP | |||||||
|---|---|---|---|---|---|---|---|---|
| 0 | 5 | 10 | 50 | 100 | 500 | 1000 | ||
|
aTc (ng/ml) or luxI.CFP | 0 | |||||||
| 1 | ||||||||
| 5 | ||||||||
| 10 | ||||||||
| 20 | ||||||||
| 50 | ||||||||
|
These values give us a clear idea of the equivalence between luxI.CFP and luxI.YFP. These values are those obtained from the microscopy experiments. A scatter plot with each dot representing a cell was also plotted to see the variation of fluorescence with the change in aTc and IPTG concentrations.
|
