July 6
From 2007.igem.org
(Difference between revisions)
(→Analysis) |
(→Analysis) |
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IPTG (uM) or luxR.YFP | IPTG (uM) or luxR.YFP | ||
|-align="center" | |-align="center" | ||
| - | | 0 | + | |0 |
| - | | 5 | + | |5 |
| - | | 10 | + | |10 |
| - | | 50 | + | |50 |
| - | | 100 | + | |100 |
| - | | 500 | + | |500 |
|1000 | |1000 | ||
|-align="center" | |-align="center" | ||
| Line 163: | Line 163: | ||
{|align="center" width="35%" | {|align="center" width="35%" | ||
|- | |- | ||
| - | |align="justify"| | + | |align="justify"|These values, obtained from the microscopy experiments, give us a clear idea of the equivalence between luxI.CFP and luxI.YFP. |
| - | These values give us a clear idea of the equivalence between luxI.CFP and luxI.YFP | + | |
| - | + | ||
| - | + | ||
A scatter plot with each dot representing a cell was also plotted to see the variation of fluorescence with the change in aTc and IPTG concentrations. | A scatter plot with each dot representing a cell was also plotted to see the variation of fluorescence with the change in aTc and IPTG concentrations. | ||
| - | {|align="center | + | {|align="center" |
|- | |- | ||
| - | |*Red : 0 ng/ml aTc | + | | |
| - | |*Green : 1 ng/ml aTc | + | *Red : 0 ng/ml aTc |
| + | | | ||
| + | *Green : 1 ng/ml aTc | ||
|- | |- | ||
| - | |*Blue : 5 ng/ml aTc | + | | |
| - | |*Cyan : 10 ng/ml aTc | + | *Blue : 5 ng/ml aTc |
| + | | | ||
| + | *Cyan : 10 ng/ml aTc | ||
|- | |- | ||
| - | |*Magenta : 20 ng/ml aTc | + | | |
| - | |*Yellow : 50 ng/ml aTc | + | *Magenta : 20 ng/ml aTc |
| + | | | ||
| + | *Yellow : 50 ng/ml aTc | ||
|} | |} | ||
Revision as of 05:39, 7 July 2007
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Experiments
Analysis
Today we compiled the data collected from all our open-loop experiments performed at various concentrations of aTc (ng/ml). The following matrix of information was obtained:
|
CFP mean values |
IPTG (uM) or luxR.YFP | |||||||
|---|---|---|---|---|---|---|---|---|
| 0 | 5 | 10 | 50 | 100 | 500 | 1000 | ||
|
aTc (ng/ml) or luxI.CFP | 0 | |||||||
| 1 | ||||||||
| 5 | ||||||||
| 10 | ||||||||
| 20 | ||||||||
| 50 | ||||||||
|
YFP mean values |
IPTG (uM) or luxR.YFP | |||||||
|---|---|---|---|---|---|---|---|---|
| 0 | 5 | 10 | 50 | 100 | 500 | 1000 | ||
|
aTc (ng/ml) or luxI.CFP | 0 | |||||||
| 1 | ||||||||
| 5 | ||||||||
| 10 | ||||||||
| 20 | ||||||||
| 50 | ||||||||
| These values, obtained from the microscopy experiments, give us a clear idea of the equivalence between luxI.CFP and luxI.YFP.
A scatter plot with each dot representing a cell was also plotted to see the variation of fluorescence with the change in aTc and IPTG concentrations.
|
