July 6
From 2007.igem.org
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| - | *Red : 0 ng/ml aTc | + | *<font color="Red">Red</font> : 0 ng/ml aTc |
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| - | *Green : 1 ng/ml aTc | + | *<font color="Green">Green</font> : 1 ng/ml aTc |
|- | |- | ||
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| - | *Blue : 5 ng/ml aTc | + | *<font color="Blue">Blue</font> : 5 ng/ml aTc |
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| - | *Cyan : 10 ng/ml aTc | + | *<font color="Cyan">Cyan</font> : 10 ng/ml aTc |
|- | |- | ||
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| - | *Magenta : 20 ng/ml aTc | + | *<font color="Magenta">Magenta</font> : 20 ng/ml aTc |
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| - | *Yellow : 50 ng/ml aTc | + | *<font color="Yellow">Yellow</font> : 50 ng/ml aTc |
| + | |} | ||
| + | |||
| + | |||
| + | {|align="justify" | ||
| + | |- | ||
| + | |[[Image:Openloop_cfp_jul6.jpg|300px|CFP fluorescence]] | ||
| + | |[[Image:Openloop_yfp_jul6.jpg|300px|YFP fluorescence]] | ||
|} | |} | ||
Revision as of 05:45, 7 July 2007
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Experiments
Analysis
Today we compiled the data collected from all our open-loop experiments performed at various concentrations of aTc (ng/ml). The following matrix of information was obtained:
|
CFP mean values |
IPTG (uM) or luxR.YFP | |||||||
|---|---|---|---|---|---|---|---|---|
| 0 | 5 | 10 | 50 | 100 | 500 | 1000 | ||
|
aTc (ng/ml) or luxI.CFP | 0 | |||||||
| 1 | ||||||||
| 5 | ||||||||
| 10 | ||||||||
| 20 | ||||||||
| 50 | ||||||||
|
YFP mean values |
IPTG (uM) or luxR.YFP | |||||||
|---|---|---|---|---|---|---|---|---|
| 0 | 5 | 10 | 50 | 100 | 500 | 1000 | ||
|
aTc (ng/ml) or luxI.CFP | 0 | |||||||
| 1 | ||||||||
| 5 | ||||||||
| 10 | ||||||||
| 20 | ||||||||
| 50 | ||||||||
| These values, obtained from the microscopy experiments, give us a clear idea of the equivalence between luxI.CFP and luxI.YFP.
A scatter plot with each dot representing a cell was also plotted to see the variation of fluorescence with the change in aTc and IPTG concentrations.
|
