Boston University/Project Progress

From 2007.igem.org

(Difference between revisions)
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[[Boston_University/Primer Design | Primer Design]]
[[Boston_University/Primer Design | Primer Design]]
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[[Boston_University/Shewy Competence and Transformation| Making Shewanella Competent and Transforming Plasmid]]
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[[Boston_University/Shewy Competence and Transformation | Making Shewanella Competent and Transforming Plasmid]]
== Materials We Need ==
== Materials We Need ==
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EDIT THE WIIIIKIIII
EDIT THE WIIIIKIIII
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[[Boston_University | Back]]
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== Protocols ==
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[[Boston_University/Heat Shock Protocol | Calcium Chloride/Heat Shock Plasmid Transformations Protocol]]
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[[Boston_University/Filter Conjugation Protocol | Filter Cojugation Protocol]]
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[[Boston_University/TOPO Protocol | pTrcHis TOPO TA Expression Kit Cloning Protocol]]
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[[Boston_University/NEBCutter Protocl| Using NEBCutter for checking specific restriction enzymes against a sequence]]
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[[Boston_University/Gel Protocol | Making an Electrophoresis Gel]]
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[[Boston_University/Zymo Protocol | Procedure for 50 mL E. Coli mixture in Zymo Broth or SOB
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]]
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== Question and Answer ==
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[[Boston_University/Project Progress | Back]]

Revision as of 17:08, 9 July 2007

Contents

What We've Accomplished

Plasmid Choice

Restriction Enzyme Choice

Primer Design

Making Shewanella Competent and Transforming Plasmid

Materials We Need

Error-Prone PCR: From CAB(?)

Ligases: Need to Buy

Short-Term To-Do List

EDIT THE WIIIIKIIII

Protocols

Calcium Chloride/Heat Shock Plasmid Transformations Protocol

Filter Cojugation Protocol

pTrcHis TOPO TA Expression Kit Cloning Protocol

Using NEBCutter for checking specific restriction enzymes against a sequence

Making an Electrophoresis Gel

Procedure for 50 mL E. Coli mixture in Zymo Broth or SOB

Question and Answer

Back