Melbourne/4 July 07 Digest
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*Method Documentation Comenced:1 July 2007 | *Method Documentation Comenced:1 July 2007 | ||
Latest revision as of 03:44, 25 October 2007
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Contents |
Dates:
- Method Documentation Comenced:1 July 2007
- Method Documentation Completed:
- Author:
- Experiment Commenced:
- Experiment Completed:10 July 2007
- Write up Completed:
Aim:
- Prepare sufficient quantities of kit and other plasmids that are intended to be used in project.
- Confirm of contents of minipreped plasmids by digest.
Method:
- Made up 25 Ampicillin plates using 400mL LB agar.(Using 100mg/ml Ampicillin stock from Gooley Lab).
- Make up Kanamycin plates.
- Resuspended the following from Registry plates.
- Transformed into Joe's competent DH5alpha cells.
- Streak the cells onto plates.
- Prepared in Amp LB (100ug/mL) or Kan LB (50mg/mL) as appropriate.
- Minipreped the cultures grown overnight after putting aside 1mL(sterile) for glycerol stocks.
- made glycerol stocks.
- Prepared an agarose gel with 1xTAE buffer.
- Digest
- Loaded 20uL of digest samples from 6/7 in the following lane order.
Results:
- Jottings:
- Gells:
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