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Revision as of 15:03, 12 July 2007 (view source) Nicolas C. (Talk | contribs) (→Preparation of a stock of phage on w121:) ← Older edit		Revision as of 15:06, 12 July 2007 (view source) Nicolas C. (Talk | contribs) (→Titration of bacteriophages P1) Newer edit →
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	* The stock prepared the 8.7.7		* The stock prepared the 8.7.7
	* The stock prepared today (12.7.7)		* The stock prepared today (12.7.7)
-	See protocols for details.	+	See [[Paris/PROTOCOLS#Titration of bacteriophages|protocols]] for details.
	== Culture of FtsZ TS ==		== Culture of FtsZ TS ==

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Revision as of 15:06, 12 July 2007

Preparation of a stock of phage on w121:

David B:

• Filtration of the Cacl2 and MgSO4 stocks
• Launch a culture:
  • 15ml LB
  • 225µl Cacl2
  • 450µl MgSO4
  • 90µl DAP
  • 150µl W121 from an ON culture
• Let grow until OD=0.2 (~1h)
• Add 100µl P1 (stock 10¹⁰/ml)
• Incubate 2h
• Add 200µl Chloroform
• Vortex, centrifuge (15min @ 3000rpm)
• Recover the supernatant + add 200µl Chloroform = Stock keep at 4°C

Titration of bacteriophages P1

Nicolas C.
We measure the strength of former P1 preparation :

• The stock prepared the 3.7.7
• The stock prepared the 8.7.7
• The stock prepared today (12.7.7)

See protocols for details.

Culture of FtsZ TS

David B
We want to isolate a good clone of the strain FstZ TS
From the plate of FtsZ TS84 121:
isolation of 6 clones of the 121.1 on LBA+tet incubated at 42°C + Culture ON at 30°C
If one clone do not grow at 42, we'll use it to do the transduction