Alberta/Calender/July
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Purification of gel slice of digested J61003. | Purification of gel slice of digested J61003. | ||
| - | -ED | + | -ED, JG, AL, JB, MC, CZ |
[[Alberta/Calender/July#July|to the top]] | [[Alberta/Calender/July#July|to the top]] | ||
Revision as of 02:44, 13 July 2007
July
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To August 2007
Back to UofA iGEM Home
July 1
July 2
July 3
July 4
Lab Notes:
Transformations of: J61003 (vector containing GFP), J23119 (constitutive promotor), E1010 (RFP), J45100 (methyl salicylate), 0034 (RBS). Transform into XL10 gold cells. Plate on Amp.
-ED, JG, ML, MC, VH, AL, CZ
July 5
July 6
Lab Notes:
Transformation of E1010 did not work, it is in a Kan vector. SMRT.
Set up 4X5mL overnights of pSB1A3, J45100, B0034, J61003.
-ED
July 7
Lab Notes:
Glycerol stocks of overnights from July 6. Stored in -80 freezer. Miniprep from overnights started July 6. Stored in "iGEM" freezer box in -20 freezer.
-JP,JB,AL
July 8
Lab Notes:
Transform E1010 and A0500 into XL10 Gold. Plate on Kan.
Pour Kan and Amp plates. Make TBE and TAE buffer.
-ED, JP, MC, VH, CZ
July 9
Lab Notes:
Retransform E1010 and A0050 into XL10Gold cells using new plates that were poured Sunday.
-ED, VH, JG
July 10
Lab Notes:
Transformations of E1010 and A0050 were not successful. Tomorrow we will try with a new cell line and enriched media.
-ED, MC, AF, CZ
July 11
Lab Notes:
Third attempt at transforming the Kan resistant BioBricks. Transformed E1010, A0500, and pSB2K3 into XL10 Gold, XL10 Gold with enriched media, and DH5a cells. Plated 100uL and the pellet remaining after taking the 100uL aliquot on Kan plates.
Restriction digest of J61003 with Xba and Spe. Run on 0.8% agarose gel. Cut out 2290 band, will gel extract tomorrow.
-ED, MC, NG, JG, VH, CZ
July 12
General Notes:
Calender up and running.
Meeting tonight at 7:00 in CSC 2-49.
Received Chlorobium tepidum, meeting with Dr. Jeff Fuller (with Capital Health) to discuss the use of their anaerobic chambers!! -Justin
Lab Notes:
Transformations with DH5a cells worked, but we will need to select for single colonies tonight.
Streak for single colonies of E1010, A0500.
Purification of gel slice of digested J61003.
-ED, JG, AL, JB, MC, CZ
July 13
July 14
July 15
July 16
July 17
July 18
July 19
Meeting at 7:00, Room TBA.
July 20
July 21
July 22
July 23
July 24
July 25
July 26
Meeting at 7:00, Room TBA.
July 27
July 28
July 29
July 30
July 31
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