BerkiGEM2007-KristinConstructionFile6
From 2007.igem.org
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Composite part of RBS (pBca1101-I716051-kan cassette) + hemA from R.capsulatus | Composite part of RBS (pBca1101-I716051-kan cassette) + hemA from R.capsulatus | ||
Digest pBca1101-I716051 (EcoR1/BamH1, 2904+1065, S) | Digest pBca1101-I716051 (EcoR1/BamH1, 2904+1065, S) | ||
Sub into pBca9145-I716151 (EcoR1/Bg1II, 3275+9, L) | Sub into pBca9145-I716151 (EcoR1/Bg1II, 3275+9, L) | ||
Product is pBca9145-I716156 | Product is pBca9145-I716156 | ||
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Revision as of 17:08, 17 July 2007
Composite part of RBS (pBca1101-I716051-kan cassette) + hemA from R.capsulatus Digest pBca1101-I716051 (EcoR1/BamH1, 2904+1065, S) Sub into pBca9145-I716151 (EcoR1/Bg1II, 3275+9, L) Product is pBca9145-I716156
