BerkiGEM2007-KristinConstructionFile24
From 2007.igem.org
(Difference between revisions)
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<pre> | <pre> | ||
Composite part of hemA from R.capsulatus/hemB/hemC/hemD + T7 promoter | Composite part of hemA from R.capsulatus/hemB/hemC/hemD + T7 promoter | ||
| - | Digest pBca9145-I716105 | + | Digest pBca9145-I716105 (EcoR1/BamHI, 6103+1027, L) |
Sub into pBca9145-I716172 (EcoR1/Bg1II, 6103+1027, L) | Sub into pBca9145-I716172 (EcoR1/Bg1II, 6103+1027, L) | ||
Product is pBca9145-I716174 | Product is pBca9145-I716174 | ||
</pre> | </pre> | ||
Latest revision as of 17:58, 17 July 2007
Composite part of hemA from R.capsulatus/hemB/hemC/hemD + T7 promoter Digest pBca9145-I716105 (EcoR1/BamHI, 6103+1027, L) Sub into pBca9145-I716172 (EcoR1/Bg1II, 6103+1027, L) Product is pBca9145-I716174
