Paris/Freezer

From 2007.igem.org

(Difference between revisions)
(→Digestion Products)
(→Plasmids: MiniPrep Products)
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|- style="background: #cccccc;"   
|- style="background: #cccccc;"   
| style="background: #ccffcc;" |1
| style="background: #ccffcc;" |1
-
|<bbpart>pJ23100</bbpart>
+
|<bbpart>pJ23100</bbpart> in <bbpart>J61002</bbpart>
|Strong constitutive promoter in J61002 (clones 1 + 2)
|Strong constitutive promoter in J61002 (clones 1 + 2)
|- style="background: #cccccc;"   
|- style="background: #cccccc;"   
| style="background: #ccffcc;" |2
| style="background: #ccffcc;" |2
-
|<bbpart>pJ23107</bbpart>
+
|<bbpart>pJ23107</bbpart> in <bbpart>J61002</bbpart>
|Medium constitutive promoter in J61002 (clones 1 + 2)
|Medium constitutive promoter in J61002 (clones 1 + 2)
|- style="background: #cccccc;"   
|- style="background: #cccccc;"   
| style="background: #ccffcc;" |3
| style="background: #ccffcc;" |3
-
|<bbpart>B0015</bbpart>
+
|<bbpart>B0015</bbpart> in <bbpart>pSB1AK3</bbpart>
-
|Double terminator in pSB1AK3 (clones 1 + 2)
+
|Double terminator (clones 1 + 2)
|- style="background: #cccccc;"   
|- style="background: #cccccc;"   
-
| style="background: #ccffcc;" |3
+
| style="background: #ccffcc;" |4
-
|<bbpart>I0500</bbpart>
+
|<bbpart>I0500</bbpart> in <bbpart>pSB2K3</bbpart>
-
|AraC pBAD in pSB2K3 (clones 1 + 2)
+
|AraC pBAD (clones 1 + 2)
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |5
 +
|<bbpart>BBa_B0030</bbpart> in <bbpart>pSB1A2</bbpart>
 +
|Strong RBS (clones 1 + 2)
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |6
 +
|<bbpart>BBa_E0422</bbpart> in <bbpart>pSB1A2</bbpart>
 +
|ECFP (RBS+LVA+Term) (clones 1 + 2)
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |7
 +
|<bbpart>BBa_E0241</bbpart> in <bbpart>pSB1A2</bbpart>
 +
|PoPs to GFP converter (clones 1 + 2)
|}
|}
 +
 +
*  <bbpart>BBa_E0840</bbpart> gfp tri-part; strong rbs (well 16E plate 1)
 +
*  <bbpart>BBa_J61047</bbpart> pSB1A2 : Cre ORF (well 8P plate 4)
== Digestion Products ==
== Digestion Products ==

Revision as of 17:15, 18 July 2007

Contents

PCR products

PCR products
Number Name Length Comments
1 Lox71-FtsA-FtsZ-1 1537
2 FtsZ-2 1088
3 DGAT-1
4 DGAT-2
5 Lox66-DapAColi 985
6 Lox66-DapASubtilis 979
7 Lox71-FtsZ 1260 EcoR1 site not mutated

Plasmids: MiniPrep Products

Plasmids: MiniPrep Products
Number Name Description
1 pJ23100 in J61002 Strong constitutive promoter in J61002 (clones 1 + 2)
2 pJ23107 in J61002 Medium constitutive promoter in J61002 (clones 1 + 2)
3 B0015 in pSB1AK3 Double terminator (clones 1 + 2)
4 I0500 in pSB2K3 AraC pBAD (clones 1 + 2)
5 BBa_B0030 in pSB1A2 Strong RBS (clones 1 + 2)
6 BBa_E0422 in pSB1A2 ECFP (RBS+LVA+Term) (clones 1 + 2)
7 BBa_E0241 in pSB1A2 PoPs to GFP converter (clones 1 + 2)
  • BBa_E0840 gfp tri-part; strong rbs (well 16E plate 1)
  • BBa_J61047 pSB1A2 : Cre ORF (well 8P plate 4)

Digestion Products

Digestion Products
Number Name Enzyme 1 Enzyme 2 Description
1 pJ23100 EcoR1 Spe1
2 pJ23100 Spe1 Pst1
3 pJ23107 EcoR1 Spe1
4 pJ23107 Spe1 Pst1
5 pSB2K3 EcoR1 Pst1
6 pSB1AK3 EcoR1 Pst1
6 Lox66 DapA E.coli (PCR product) EcoR1 Pst1
7 Lox66 DapA E.coli (PCR product) Xba1 Pst1

Strains (glycerol stock)

  • MG1655
  • Acinetobacter ADP1
  • w121
  • p2C (CFP fluorescent)
  • p2Y (YFP fluorescent)
  • FtsZTS strains
    • MG1655 FtsZ -TS 84
    • 121.1
    • 121.2
    • 121.3
    • 121.4
    • 121.5
    • 121.6
    • 129
    • 1.129
    • DCR 14.1
    • DCR 14.2
    • DCR 14.3
  • DH5alpha pKs::DGAT
  • pJ23100 Clone1
  • pJ23100 Clone2
  • BBa_B0015 Clone1
  • BBa_B0015 Clone2
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