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Revision as of 17:58, 18 July 2007 (view source) David.bikard (Talk | contribs) (→Digestion Products) ← Older edit		Revision as of 18:06, 18 July 2007 (view source) David.bikard (Talk | contribs) (→Digestion Products) Newer edit →
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	|width=5%| Number		|width=5%| Number
	|width=25%| Name		|width=25%| Name
		+	|width=25%| Matrix
	|width=5%| Enzyme 1		|width=5%| Enzyme 1
	|width=5%| Enzyme 2		|width=5%| Enzyme 2
-	|width=55%| Description	+	|width=30%| Description
	|width=5%| Date		|width=5%| Date
	|- style="background: #cccccc;"		|- style="background: #cccccc;"
	| style="background: #ccffcc;" |D1		| style="background: #ccffcc;" |D1
	|<bbpart>pJ23100</bbpart>		|<bbpart>pJ23100</bbpart>
		+	|
	|EcoR1		|EcoR1
	|Spe1		|Spe1
Line 130:		Line 132:
	| style="background: #ccffcc;" |D2		| style="background: #ccffcc;" |D2
	|<bbpart>pJ23100</bbpart>		|<bbpart>pJ23100</bbpart>
		+	|
	|Spe1		|Spe1
	|Pst1		|Pst1
Line 137:		Line 140:
	| style="background: #ccffcc;" |3		| style="background: #ccffcc;" |3
	|<bbpart>pJ23107</bbpart>		|<bbpart>pJ23107</bbpart>
		+	|
	|EcoR1		|EcoR1
	|Spe1		|Spe1
Line 144:		Line 148:
	| style="background: #ccffcc;" |4		| style="background: #ccffcc;" |4
	|<bbpart>pJ23107</bbpart>		|<bbpart>pJ23107</bbpart>
		+	|
	|Spe1		|Spe1
	|Pst1		|Pst1
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	| style="background: #ccffcc;" |5		| style="background: #ccffcc;" |5
	|<bbpart>pSB2K3</bbpart>		|<bbpart>pSB2K3</bbpart>
		+	|
	|EcoR1		|EcoR1
	|Pst1		|Pst1
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	| style="background: #ccffcc;" |6		| style="background: #ccffcc;" |6
	|<bbpart>pSB1AK3</bbpart>		|<bbpart>pSB1AK3</bbpart>
		+	|
	|EcoR1		|EcoR1
	|Pst1		|Pst1
Line 165:		Line 172:
	| style="background: #ccffcc;" |6		| style="background: #ccffcc;" |6
	|Lox66 DapA E.coli (PCR product)		|Lox66 DapA E.coli (PCR product)
		+	|
	|EcoR1		|EcoR1
	|Pst1		|Pst1
Line 172:		Line 180:
	| style="background: #ccffcc;" |7		| style="background: #ccffcc;" |7
	|Lox66 DapA E.coli (PCR product)		|Lox66 DapA E.coli (PCR product)
		+	|
	|Xba1		|Xba1
	|Pst1		|Pst1

---

Revision as of 18:06, 18 July 2007

Contents 1 PCR products 2 Plasmids: MiniPrep Products 3 Digestion Products 4 Strains (glycerol stock)

PCR products

PCR products
Number	Name	Length	Comments	Date
1	Lox71-FtsA-FtsZ-1	1537
2	FtsZ-2	1088
3	DGAT-1
4	DGAT-2
5	Lox66-DapAColi	985
6	Lox66-DapASubtilis	979		17/07/07
7	Lox71-FtsZ	1260	EcoR1 site not mutated	17/07/07

Plasmids: MiniPrep Products

Plasmids: MiniPrep Products
Number	Name	Description	Date
1	pJ23100 in J61002	Strong constitutive promoter in J61002 (clones 1 + 2)	15/07/07
2	pJ23107 in J61002	Medium constitutive promoter in J61002 (clones 1 + 2)	15/07/07
3	B0015 in pSB1AK3	Double terminator (clones 1 + 2)	16/07/07
4	I0500 in pSB2K3	AraC pBAD (clones 1 + 2)	16/07/07
5	BBa_B0030 in pSB1A2	Strong RBS (clones 1 + 2)	18/07/07
6	BBa_E0422 in pSB1A2	ECFP (RBS+LVA+Term) (clones 1 + 2)	18/07/07
7	BBa_E0241 in pSB1A2	PoPs to GFP converter (clones 1 + 2)	18/07/07
8	BBa_E0840 in pSB1A2	gfp tri-part; strong rbs (clones 1 + 2)	18/07/07
9	BBa_J61047 in pSB1A2	Cre ORF (clones 1 + 2)	18/07/07

Digestion Products

Digestion Products
Number	Name	Matrix	Enzyme 1	Enzyme 2	Description	Date
D1	pJ23100		EcoR1	Spe1
D2	pJ23100		Spe1	Pst1
3	pJ23107		EcoR1	Spe1
4	pJ23107		Spe1	Pst1
5	pSB2K3		EcoR1	Pst1
6	pSB1AK3		EcoR1	Pst1
6	Lox66 DapA E.coli (PCR product)		EcoR1	Pst1
7	Lox66 DapA E.coli (PCR product)		Xba1	Pst1

Strains (glycerol stock)

• MG1655
• Acinetobacter ADP1
• w121
• p2C (CFP fluorescent)
• p2Y (YFP fluorescent)
• FtsZ^TS strains
  • MG1655 FtsZ -TS 84
  • 121.1
  • 121.2
  • 121.3
  • 121.4
  • 121.5
  • 121.6
  • 129
  • 1.129
  • DCR 14.1
  • DCR 14.2
  • DCR 14.3
• DH5alpha pKs::DGAT
• pJ23100 Clone1
• pJ23100 Clone2
• BBa_B0015 Clone1
• BBa_B0015 Clone2