Edinburgh/Lab
From 2007.igem.org
(Difference between revisions)
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==Lab Diary== | ==Lab Diary== | ||
| - | Thu 12th July | + | ====Thu 12th July==== |
| - | + | * PTG induced GFP revived ([http://partsregistry.org/Part:BBa_J04430 BBa_J04430]) | |
| - | :: Test biobrick revival process | + | ::Test biobrick revival process |
| - | Fri 13th July | + | ====Fri 13th July==== |
| - | + | * Alfalfa Seeds Planted (in Agar) | |
| - | :: Growing alfalfa plants, to get leaves from which to extract DNA for the caffeic acid-3-o-methyltransferase gene (part of the vanillin synthesis pathway) | + | ::Growing alfalfa plants, to get leaves from which to extract DNA for the caffeic acid-3-o-methyltransferase gene (part of the vanillin synthesis pathway) |
| - | + | * Biobricks [http://partsregistry.org/Part:BBa_E0241 BBa_E0241] and [http://partsregistry.org/Part:BBa_J45700 BBa_J45700] rivived | |
| - | Mon 16th July | + | ====Mon 16th July==== |
| - | + | * Begin DNA extraction from Bamboo | |
| - | :: Test DNA extraction process from plants | + | ::Test DNA extraction process from plants |
| - | + | * Prepared mini preps for GFP and mint genes | |
| - | Tue 17th July | + | ====Tue 17th July==== |
| - | + | * Alfalfa Transferred to compost | |
| - | + | * DNA containing GFP and mint genes extracted from E.Coli | |
| + | |||
| + | ====Wed 18th July==== | ||
| + | * Digest GFP and mint mini preps and ran on gel | ||
Revision as of 10:09, 19 July 2007
https://static.igem.org/mediawiki/2007/f/f5/800px-Edinburgh_City_15_mod.JPG
Contents |
Biobricks Revived
This is a list of the biobricks we have revived from the registry
| Brick | Description | Well | Used for |
|---|---|---|---|
| [http://partsregistry.org/Part:BBa_J04430 BBa_J04430] | IPTG induced GFP | Plate 2, 1L | Test biobrick revival process |
| [http://partsregistry.org/Part:BBa_E0241 BBa_E0241] | PoPS --> GFP | Plate 2, 15L | For use in dif experement 1 |
| [http://partsregistry.org/Part:BBa_J45700 BBa_J45700] | Wintergreen Scent | Plate 3, 7B | Place into lacto bacillus |
Lab Diary
Thu 12th July
- PTG induced GFP revived ([http://partsregistry.org/Part:BBa_J04430 BBa_J04430])
- Test biobrick revival process
Fri 13th July
- Alfalfa Seeds Planted (in Agar)
- Growing alfalfa plants, to get leaves from which to extract DNA for the caffeic acid-3-o-methyltransferase gene (part of the vanillin synthesis pathway)
- Biobricks [http://partsregistry.org/Part:BBa_E0241 BBa_E0241] and [http://partsregistry.org/Part:BBa_J45700 BBa_J45700] rivived
Mon 16th July
- Begin DNA extraction from Bamboo
- Test DNA extraction process from plants
- Prepared mini preps for GFP and mint genes
Tue 17th July
- Alfalfa Transferred to compost
- DNA containing GFP and mint genes extracted from E.Coli
Wed 18th July
- Digest GFP and mint mini preps and ran on gel
