Paris/July 21
From 2007.igem.org
(Difference between revisions)
Eismoustique (Talk | contribs) (→Digestion reactions) |
Eismoustique (Talk | contribs) (→Digestion reactions) |
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| Line 9: | Line 9: | ||
|width=5%| Enzyme 1 | |width=5%| Enzyme 1 | ||
|width=5%| Enzyme 2 | |width=5%| Enzyme 2 | ||
| - | |width= | + | |width=5%| size |
| + | |width=35%| Description | ||
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| style="background: #ccffcc;" |D9 | | style="background: #ccffcc;" |D9 | ||
| Line 16: | Line 17: | ||
|EcoI | |EcoI | ||
|SpeI | |SpeI | ||
| + | | | ||
|Vector with mRFP RBS/CDS ready for insertion promoter (fw-insert) | |Vector with mRFP RBS/CDS ready for insertion promoter (fw-insert) | ||
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| Line 23: | Line 25: | ||
|EcoI | |EcoI | ||
|SpeI | |SpeI | ||
| + | | | ||
|J23100 promoter ready for usage as a fw-insert | |J23100 promoter ready for usage as a fw-insert | ||
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| Line 30: | Line 33: | ||
|EcoRI | |EcoRI | ||
|SpeI | |SpeI | ||
| + | | | ||
|Vector with mRFP RBS/CDS ready for insertion of a fw-insert promoter | |Vector with mRFP RBS/CDS ready for insertion of a fw-insert promoter | ||
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| Line 37: | Line 41: | ||
|EcoRI | |EcoRI | ||
|SpeI | |SpeI | ||
| + | | | ||
|J23107 promoter ready for usage as a fw-insert | |J23107 promoter ready for usage as a fw-insert | ||
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| Line 44: | Line 49: | ||
|SpeI | |SpeI | ||
|PstI | |PstI | ||
| + | | | ||
|mRFP RBS/CDS/T sequence, can it be used as a backward inser? | |mRFP RBS/CDS/T sequence, can it be used as a backward inser? | ||
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| Line 51: | Line 57: | ||
|XbaI | |XbaI | ||
|PstI | |PstI | ||
| + | | | ||
|Cre ORF extracted for usage as a bw-insert | |Cre ORF extracted for usage as a bw-insert | ||
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| Line 58: | Line 65: | ||
|SpeI | |SpeI | ||
|PstI | |PstI | ||
| + | | | ||
|bacward vector for cloning a CDS bw-insert after the B0030 RBS | |bacward vector for cloning a CDS bw-insert after the B0030 RBS | ||
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| Line 65: | Line 73: | ||
|EcoRI | |EcoRI | ||
|PstI | |PstI | ||
| + | | | ||
|pSB1AK3 open vector for BioBrick cloning | |pSB1AK3 open vector for BioBrick cloning | ||
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| Line 72: | Line 81: | ||
|EcoRI | |EcoRI | ||
|PstI | |PstI | ||
| + | | | ||
|pSB1A2 open vector for BioBrick cloning | |pSB1A2 open vector for BioBrick cloning | ||
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| Line 79: | Line 89: | ||
|EcoRI | |EcoRI | ||
|PstI | |PstI | ||
| + | | | ||
|PCR product digested for cloning as a BioBrick | |PCR product digested for cloning as a BioBrick | ||
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| Line 86: | Line 97: | ||
|EcoRI | |EcoRI | ||
|PstI | |PstI | ||
| + | | | ||
|PCR product digested for cloning as a BioBrick | |PCR product digested for cloning as a BioBrick | ||
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| Line 93: | Line 105: | ||
|XbaI | |XbaI | ||
|PstI | |PstI | ||
| + | | | ||
|PCR product digested for usage as a forward insert | |PCR product digested for usage as a forward insert | ||
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| Line 100: | Line 113: | ||
|XbaI | |XbaI | ||
|PstI | |PstI | ||
| + | | | ||
|PCR product digested for usage as a forward insert | |PCR product digested for usage as a forward insert | ||
|} | |} | ||
Revision as of 14:04, 23 July 2007
Digestion reactions
| Digestion Products | ||||||
|---|---|---|---|---|---|---|
| Number | Product Name | Matrix Name | Enzyme 1 | Enzyme 2 | size | Description |
| D9 | pJ23100 ES vector | pJ23100 (MP1.1 & MP1.2) | EcoI | SpeI | Vector with mRFP RBS/CDS ready for insertion promoter (fw-insert) | |
| D10 | pJ23100 promoter fw-insert | pJ23100 (MP1.1 & MP1.2) | EcoI | SpeI | J23100 promoter ready for usage as a fw-insert | |
| D11 | pJ23107 ES vector | pJ23107 (MP2.1, MP2.2, MP2.3 & MP2.4) | EcoRI | SpeI | Vector with mRFP RBS/CDS ready for insertion of a fw-insert promoter | |
| D12 | pJ23107 promoter fw-insert | pJ23107 (MP2.1, MP2.2, MP2.3 & MP2.4) | EcoRI | SpeI | J23107 promoter ready for usage as a fw-insert | |
| D13 | mRFP | pJ23107 (MP2.2, MP2.3 & MP2.4) | SpeI | PstI | mRFP RBS/CDS/T sequence, can it be used as a backward inser? | |
| D14 | Cre ORF | J61047 MP9.1 & MP9.2 | XbaI | PstI | Cre ORF extracted for usage as a bw-insert | |
| D15 | B0030 bw-vector | B0030 MP5.1 & MP5.2 | SpeI | PstI | bacward vector for cloning a CDS bw-insert after the B0030 RBS | |
| D16 | pSB1AK3 vector | B0015 MP3.1 & MP3.2 | EcoRI | PstI | pSB1AK3 open vector for BioBrick cloning | |
| D17 | pSB1A2 E0422 vector | E0422 (MP6.1 & MP6.2) | EcoRI | PstI | pSB1A2 open vector for BioBrick cloning | |
| D18 | BB dig. lox66DapAE.coli | lox66-DapAE.coli P5 PCR product | EcoRI | PstI | PCR product digested for cloning as a BioBrick | |
| D19 | BB dig. lox66DapAsubtilis | lox66-DapAsubtilis P6 PCR product | EcoRI | PstI | PCR product digested for cloning as a BioBrick | |
| D20 | fw insert lox66-DapAE.coli | lox66-DapAE.coli P5 PCR product | XbaI | PstI | PCR product digested for usage as a forward insert | |
| D21 | fw insert lox66-DapAsubtilis | lox66-DapAsubtilis P6 PCR product | XbaI | PstI | PCR product digested for usage as a forward insert | |
