Boston University/Plasmid Customization
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+ | ::::'''Verifying the presence of the lacZ promoter in plasmid pJQ200''' | ||
+ | :The image immediately to the right of this text shows the results from our transformation of E.coli with plasmids pBAD18s, pBAD30, and pjQ200 via Zymo transformation and electroporation. | ||
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+ | :The contents of the plates are as follows(column indicates transformation type): | ||
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+ | |'''Zymo(poured)'''||'''Zymo(streaked)'''||'''Electroporation''' | ||
+ | |- | ||
+ | |pBAD18s|||pBAD18s|||pBAD18s | ||
+ | |- | ||
+ | |pBAD30|||pBAD30|||pBAD30 | ||
+ | |- | ||
+ | |pjQ200|||pjQ200|||pjQ200 | ||
+ | |} | ||
+ | |||
[[Image:BU_labeled_lacz_PCR_cropped_7-17.jpg]] | [[Image:BU_labeled_lacz_PCR_cropped_7-17.jpg]] | ||
[[Image:BU_labeled_digested_pjq200_7-19-2007.jpg]] | [[Image:BU_labeled_digested_pjq200_7-19-2007.jpg]] |
Revision as of 18:01, 23 July 2007
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