Boston University/Plasmid Customization

From 2007.igem.org

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::::'''Verifying the presence of the lacZ promoter in plasmid pJQ200'''
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:The image immediately to the right of this text shows the results from our transformation of E.coli with plasmids pBAD18s, pBAD30, and pjQ200 via Zymo transformation and electroporation.
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:The contents of the plates are as follows(column indicates transformation type):
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{| align="center" border="1"
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|'''Zymo(poured)'''||'''Zymo(streaked)'''||'''Electroporation'''
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|-
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|pBAD18s|||pBAD18s|||pBAD18s
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|-
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|pBAD30|||pBAD30|||pBAD30
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|-
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|pjQ200|||pjQ200|||pjQ200
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|}
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[[Image:BU_labeled_lacz_PCR_cropped_7-17.jpg]]
[[Image:BU_labeled_lacz_PCR_cropped_7-17.jpg]]
[[Image:BU_labeled_digested_pjq200_7-19-2007.jpg]]
[[Image:BU_labeled_digested_pjq200_7-19-2007.jpg]]

Revision as of 18:01, 23 July 2007

Verifying the presence of the lacZ promoter in plasmid pJQ200
The image immediately to the right of this text shows the results from our transformation of E.coli with plasmids pBAD18s, pBAD30, and pjQ200 via Zymo transformation and electroporation.
The contents of the plates are as follows(column indicates transformation type):
Zymo(poured)Zymo(streaked)Electroporation
pBAD18spBAD18spBAD18s
pBAD30pBAD30pBAD30
pjQ200pjQ200pjQ200

BU labeled lacz PCR cropped 7-17.jpg BU labeled digested pjq200 7-19-2007.jpg BU labeled lacz PCR 7-19-2007.jpg BU labeled lacz PCR 7-21-2007.jpg BU labeled pjq200 sacb digestion 7-17.jpg