Synthesis of First Bacterial Computer
From 2007.igem.org
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The first experiment is to control that we will be able to see GFP and RFP at the same time in bacterial colonies | The first experiment is to control that we will be able to see GFP and RFP at the same time in bacterial colonies | ||
| - | Plac:RBS:RFP:GFP | + | Plac:RBS:RFP:RBS:GFP |
The second experiment is: if the problem is solved, will the construct show the desired phenotype. | The second experiment is: if the problem is solved, will the construct show the desired phenotype. | ||
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T7:RBS:RFP1:hix:RFP2:RBS:GFP1:hix:GFP2 | T7:RBS:RFP1:hix:RFP2:RBS:GFP1:hix:GFP2 | ||
| - | The third deals with making sure that | + | The third deals with making sure that a polypeptide synthesized with different halves of the two different genes (becuase they are both flouresent proteins) does not produce a postive phenotype. |
Plac:RBS:GFP1:hix:RFP2 | Plac:RBS:GFP1:hix:RFP2 | ||
Revision as of 18:01, 23 July 2007
Now that we have an idea of which genes are “splittable” and therefore a number of nodes that we are able to work with, we can begin to build our first bacterial computer. There are three control experiments that our group has decided to conduct in conjunction with the construction of the bacterial computer.
The first experiment is to control that we will be able to see GFP and RFP at the same time in bacterial colonies
Plac:RBS:RFP:RBS:GFP
The second experiment is: if the problem is solved, will the construct show the desired phenotype.
T7:RBS:RFP1:hix:RFP2:RBS:GFP1:hix:GFP2
The third deals with making sure that a polypeptide synthesized with different halves of the two different genes (becuase they are both flouresent proteins) does not produce a postive phenotype.
Plac:RBS:GFP1:hix:RFP2 and Plac:RBS:RFP1:hix:GFP2
In conjunction with these controls we will synthesize our first bacterial computer
T7 RFP1-hix-[GFP2-RFP1]-hix-[GFP2-TT]-hix-[RFP2-GFP1]-hix
Note: The first half of each gene is ligated to an RBS
Note: We will lastly be testing the T7 promoter, and the UV5 hybrid pLac promoter. The second control experiment will also function as a second promoter tester and we plan on comparing results from the three promoter testers (Davidson’s PT, MW PT, and the newest PT) to discern which method of controlling gene expression we will use.
