Paris/July 25

From 2007.igem.org

(Difference between revisions)
(→=Digestions)
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                                             EcoRI & PstI for BioBricks
                                             EcoRI & PstI for BioBricks
* P10 (Lox71-FtsA-FtsZ) with XbaI & PstI for BW insert
* P10 (Lox71-FtsA-FtsZ) with XbaI & PstI for BW insert
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 +
 +
{|
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|- style="background: #ccccff;"
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! colspan="5" style="background: #ccccff;" | Digestion Products
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|- style="background: #ccccff; text-align: center;"
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|width=5%| Number
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|width=20%| Product Name
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|width=30%| Matrix Name
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|width=5%| Enzyme 1
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|width=5%| Enzyme 2
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|width=5%| size
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|width=35%| Description
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|- style="background: #cccccc;" 
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| style="background: #ccffcc;" |D22
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|pSB1A2 open vector
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|<bbpart>BBa_J61047</bbpart> (MP9.1 & MP9.2)
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|EcoI
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|PstI
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|
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|Open pSB1A2 vector for BioBrick EcoRI/PstI insertio
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|
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|
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|- style="background: #cccccc;" 
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| style="background: #ccffcc;" |D23
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|AraC/pBad promoter fw-insert
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|<bbpart>BBa_I0500</bbpart> (MP4.1 & MP4.2)
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|EcoI
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|SpeI
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|
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|AraC/pBad promoter ready for use as a forward insert
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|
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|
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|- style="background: #cccccc;" 
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| style="background: #ccffcc;" |D24
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|eCFP bw-insert
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|<bbpart>BBa_E0422</bbpart> (MP6.1 & MP6.2)
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|XbaI
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|PstI
 +
|
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|eCFP (RBS-OFR_LVA-T) ready for use as a backward insert
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|
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|
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|- style="background: #cccccc;" 
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| style="background: #ccffcc;" |D25
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|PoPs to GFP converter
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|<bbpart>BBa_E0421</bbpart> (MP7.1 & MP7.2)
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|XbaI
 +
|PstI
 +
|
 +
|PoPs to GFP converter ready for use as a backward insert
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|
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|
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|- style="background: #cccccc;" 
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| style="background: #ccffcc;" |D26
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|gfp-tripart
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|<bbpart>BBa_E0840</bbpart> (MP8.1 & MP8.2)
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|XbaI
 +
|PstI
 +
|
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|gfp-tri part (strongRBS-ORF-T) ready for use as a backward insert
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|
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|
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|- style="background: #cccccc;" 
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| style="background: #ccffcc;" |D27
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|lox71-ftsZ bw-insert
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|lox71-ftsZ PCR product P7
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|XbaI
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|PstI
 +
|
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|lox71-ftsZ(unmutated) ready for use as a backward insert
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|
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|
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|}

Revision as of 10:35, 25 July 2007

=Digestions

  • P8 (RBS-DapAColi) with XbaI & PstI for BW insert 
                                      EcoRI & PstI for BioBricks
  • P9 (RBS-DapASubtilis) with XbaI & PstI for BW insert 
                                            EcoRI & PstI for BioBricks
  • P10 (Lox71-FtsA-FtsZ) with XbaI & PstI for BW insert


Digestion Products
Number Product Name Matrix Name Enzyme 1 Enzyme 2 size Description
D22 pSB1A2 open vector BBa_J61047 (MP9.1 & MP9.2) EcoI PstI Open pSB1A2 vector for BioBrick EcoRI/PstI insertio
D23 AraC/pBad promoter fw-insert BBa_I0500 (MP4.1 & MP4.2) EcoI SpeI AraC/pBad promoter ready for use as a forward insert
D24 eCFP bw-insert BBa_E0422 (MP6.1 & MP6.2) XbaI PstI eCFP (RBS-OFR_LVA-T) ready for use as a backward insert
D25 PoPs to GFP converter BBa_E0421 (MP7.1 & MP7.2) XbaI PstI PoPs to GFP converter ready for use as a backward insert
D26 gfp-tripart BBa_E0840 (MP8.1 & MP8.2) XbaI PstI gfp-tri part (strongRBS-ORF-T) ready for use as a backward insert
D27 lox71-ftsZ bw-insert lox71-ftsZ PCR product P7 XbaI PstI lox71-ftsZ(unmutated) ready for use as a backward insert
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