Paris/July 25
From 2007.igem.org
(Difference between revisions)
(→Digestions) |
(→Digestions) |
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| Line 19: | Line 19: | ||
|PstI | |PstI | ||
|~950 | |~950 | ||
| - | | | + | |Biobrick |
| | | | ||
| | | | ||
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| style="background: #ccffcc;" |D29 | | style="background: #ccffcc;" |D29 | ||
| - | |RBS-DapAColi | + | |RBS-DapAColi |
|P8 (RBS-DapAColi) | |P8 (RBS-DapAColi) | ||
|XbaI | |XbaI | ||
|PstI | |PstI | ||
|~950 | |~950 | ||
| - | | | + | |Backward insert |
| | | | ||
| | | | ||
| Line 39: | Line 39: | ||
|PstI | |PstI | ||
|~950 | |~950 | ||
| - | | | + | |Biobrick |
| | | | ||
| | | | ||
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| style="background: #ccffcc;" |D31 | | style="background: #ccffcc;" |D31 | ||
| - | |RBS-DapASubtilis | + | |RBS-DapASubtilis |
|P9 (RBS-DapASubtilis) | |P9 (RBS-DapASubtilis) | ||
|XbaI | |XbaI | ||
|PstI | |PstI | ||
|~950 | |~950 | ||
| - | | | + | |Backward insert |
| | | | ||
| | | | ||
| Line 59: | Line 59: | ||
|PstI | |PstI | ||
|~2600 | |~2600 | ||
| - | | | + | |Backward insert |
| - | | | + | |EcoRI site not mutated : don't have to use EcoRI |
| | | | ||
|} | |} | ||
Revision as of 10:56, 25 July 2007
Digestions
| Digestion Products | ||||||||
|---|---|---|---|---|---|---|---|---|
| Number | Product Name | Matrix Name | Enzyme 1 | Enzyme 2 | size | Description | ||
| D28 | RBS-DapAColi BB | P8 (RBS-DapAColi) | EcoRI | PstI | ~950 | Biobrick | ||
| D29 | RBS-DapAColi | P8 (RBS-DapAColi) | XbaI | PstI | ~950 | Backward insert | ||
| D30 | RBS-DapASubtilis BB | P9 (RBS-DapASubtilis) | EcoRI | PstI | ~950 | Biobrick | ||
| D31 | RBS-DapASubtilis | P9 (RBS-DapASubtilis) | XbaI | PstI | ~950 | Backward insert | ||
| D32 | Lox71-FtsA-FtsZ | P10 Lox71-FtsA-FtsZ | XbaI | PstI | ~2600 | Backward insert | EcoRI site not mutated : don't have to use EcoRI | |
