Edinburgh/Lab

From 2007.igem.org

(Difference between revisions)
(Biobricks Revived)
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| [http://partsregistry.org/Part:BBa_J04430 BBa_J04430] || IPTG induced GFP || Plate 2, 1L || Test biobrick revival process
| [http://partsregistry.org/Part:BBa_J04430 BBa_J04430] || IPTG induced GFP || Plate 2, 1L || Test biobrick revival process
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|-
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| [http://partsregistry.org/Part:BBa_E0241 BBa_E0241] || PoPS --> GFP || Plate 2, 15L || For use in [https://2007.igem.org/Edinburgh/Counter#Exp_1 dif experement 1]
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| [http://partsregistry.org/Part:BBa_E0241 BBa_E0241] || PoPS --> GFP || Plate 2, 15L || For use in [https://2007.igem.org/Edinburgh/DivisionPopper#Exp_1 dif experement 1]
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|[http://partsregistry.org/Part:BBa_J45700 BBa_J45700] || Wintergreen Scent || Plate 3, 7B || Place into lacto bacillus
|[http://partsregistry.org/Part:BBa_J45700 BBa_J45700] || Wintergreen Scent || Plate 3, 7B || Place into lacto bacillus
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|-
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|[http://partsregistry.org/Part:BBa_E0422 BBa_E0422] || fast degrading ECFP || Plate 1, 11G || For use in [https://2007.igem.org/Edinburgh/Counter#Exp_1 dif experement 1]
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|[http://partsregistry.org/Part:BBa_E0422 BBa_E0422] || fast degrading ECFP || Plate 1, 11G || For use in [https://2007.igem.org/Edinburgh/DivisionPopper#Exp_1 dif experement 1]
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|}

Revision as of 12:24, 25 July 2007

https://static.igem.org/mediawiki/2007/f/f5/800px-Edinburgh_City_15_mod.JPG

Contents

Biobricks Revived

This is a list of the biobricks we have revived from the registry

Brick Description Well Used for
[http://partsregistry.org/Part:BBa_J04430 BBa_J04430] IPTG induced GFP Plate 2, 1L Test biobrick revival process
[http://partsregistry.org/Part:BBa_E0241 BBa_E0241] PoPS --> GFP Plate 2, 15L For use in dif experement 1
[http://partsregistry.org/Part:BBa_J45700 BBa_J45700] Wintergreen Scent Plate 3, 7B Place into lacto bacillus
[http://partsregistry.org/Part:BBa_E0422 BBa_E0422] fast degrading ECFP Plate 1, 11G For use in dif experement 1

Lab Diary

Thu 12th July

  • PTG induced GFP revived ([http://partsregistry.org/Part:BBa_J04430 BBa_J04430])
Test biobrick revival process

Fri 13th July

  • Alfalfa Seeds Planted (in Agar)
Growing alfalfa plants, to get leaves from which to extract DNA for the caffeic acid-3-o-methyltransferase gene (part of the vanillin synthesis pathway)
  • Biobricks [http://partsregistry.org/Part:BBa_E0241 BBa_E0241] and [http://partsregistry.org/Part:BBa_J45700 BBa_J45700] revived

Mon 16th July

  • Begin DNA extraction from Bamboo
Test DNA extraction process from plants
  • Prepared mini preps for GFP and mint genes

Tue 17th July

  • Alfalfa Transferred to compost
  • DNA containing GFP and mint genes extracted from E.Coli

Wed 18th July

  • Digest GFP and mint mini preps and ran on gel
  • Biobrick [http://partsregistry.org/Part:BBa_E0422 BBa_E0422] (ECFP) revived

Thu 19th July

  • Repeat digest of GFP and mint mini preps with more DNA

Fri 20th July

  • Prepared GFP and winter green biobricks for sequencing
  • Revived Pseudamonas Fluorescens from freeze dried culture
Used to retrieve frs and ech genes in vanillin pathway
  • Prepare 4 mini preps of ECFP

Mon 23rd July

  • Ran gels for ECFP mini preps
Digested using Xba1 and Prt1