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Revision as of 16:01, 1 August 2007 (view source) Davidpz (Talk | contribs) ← Older edit		Revision as of 16:17, 1 August 2007 (view source) Davidpz (Talk | contribs) (→DGAT Assembly PCR: Megaprimer PCR) Newer edit →
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	- Taq: 0.5µL		- Taq: 0.5µL
	- Water: 29µL		- Water: 29µL
-	- Primer: ForDGAT1 (): 4µL	+	- Primer: ForDGAT1: 4µL (final 0.8µM)
-	- Megaprimer: DGAT2: 4µL	+	- Megaprimer: DGAT2: 4µL (

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Revision as of 16:17, 1 August 2007

DGAT Assembly PCR: Megaprimer PCR

Material:

- DNA plasmid (pKS::DGAT): 1.5µL (200ng) - Tampon: 10µL - dNTP: 1µL (final 200µmol each) - Taq: 0.5µL - Water: 29µL - Primer: ForDGAT1: 4µL (final 0.8µM) - Megaprimer: DGAT2: 4µL (