Alberta/Calender/August
From 2007.igem.org
(→August 1) |
(→August 1) |
||
| Line 85: | Line 85: | ||
<b>General Notes:</b> | <b>General Notes:</b> | ||
| - | + | 1. Run gel of B0034 SpeI/PstI double digest; extract gel with QIAgen kit; stored in freezer. | |
| + | 2. PCR for | ||
| + | a. Enny + B0034 miniprep 1 to 8 | ||
| + | b. Betty colonies (10) | ||
| + | c. Buddy colonies (10) | ||
| + | Follow James' protocol. Also see blackboard for James' comment about PCR. | ||
| - | + | For tomorrow | |
| - | + | 1. run gel of PCR prodcut which are in the thermocycler � | |
| - | + | 2. check restreaked single colonies of the Betty and Buddy colony PCR | |
| - | 2 | + | 3. grow up Betty and Buddy overnight and for miniprep and ligation later |
| - | + | 4. Make Amp plates (we only have 4 more) | |
| - | 4 | + | 5. Autoclave test tubes |
| - | + | ||
| - | + | Quotes of the day: | |
| + | We are tired. | ||
[[Alberta/Calender/August#August|to the top]] | [[Alberta/Calender/August#August|to the top]] | ||
Revision as of 03:31, 2 August 2007
August
| ||||||||||||||||||||||||||||||||||||||||||||||||||
To July 2007
To September 2007
Back to UofA iGEM Home
August 1
Schedule: CZ,NG,VH
General Notes: 1. Run gel of B0034 SpeI/PstI double digest; extract gel with QIAgen kit; stored in freezer. 2. PCR for a. Enny + B0034 miniprep 1 to 8 b. Betty colonies (10) c. Buddy colonies (10) Follow James' protocol. Also see blackboard for James' comment about PCR.
For tomorrow 1. run gel of PCR prodcut which are in the thermocycler � 2. check restreaked single colonies of the Betty and Buddy colony PCR 3. grow up Betty and Buddy overnight and for miniprep and ligation later 4. Make Amp plates (we only have 4 more) 5. Autoclave test tubes
Quotes of the day: We are tired.
August 2
Modeling Meeting @ 1800hrs in CAB373
General Meeting @ 1900hrs in CAB373
Party afterward's @ Garneau pub
Schedule:
MC, JP & AF are meeting at 1700hrs to get some stuff done before the meeting happens; come join in the fun if you'd like.
August 3
Schedule:
Nik cant make it he just isn't crazy enough looks like justin is currently the only one crazy enough
Michelle & Justin are now the long weekend crew - be prepared for ILLUSIONS!!!! or perhaps just some awesome pipetting techniques while they're prepping to champ out at the ULTIMATE pipetting Olympiads.
August 4
August 5
August 6
August 7
August 8
August 9
August 10
August 11
August 12
August 13
August 14
August 15
August 16
August 17
August 18
August 19
August 20
August 21
August 22
August 23
August 24
August 25
August 26
August 27
August 28
August 29
August 30
August 31
UofA iGEM Home
To July 2007
To September 2007
