Turkey/ Possible Projects
From 2007.igem.org
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== Project 1 Mexican Wave == | == Project 1 Mexican Wave == | ||
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* Our first thought was to create a pattern by the change of colors in a concentration dependent manner; so that the production of a protein at the end of a different event would trigger the pattern formation, just like a goal triggering a *Mexican wave in soccer games. | * Our first thought was to create a pattern by the change of colors in a concentration dependent manner; so that the production of a protein at the end of a different event would trigger the pattern formation, just like a goal triggering a *Mexican wave in soccer games. | ||
* The plan was to have a plate having a lawn of same type E. coli cells having 3 constructs. Each construct would have a promoter activated by a different level of the initial signal protein and a reporter fluorescent protein coding part. | * The plan was to have a plate having a lawn of same type E. coli cells having 3 constructs. Each construct would have a promoter activated by a different level of the initial signal protein and a reporter fluorescent protein coding part. | ||
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[[Image:taslak.jpg]] | [[Image:taslak.jpg]] | ||
| + | SECOND VIEW | ||
| + | * The second view was to make this color change continious and independent of a created concentration gradient. So first idea came up to be coupling this oscillation to a already oscillating protein, maybe to cell cycle. But then this oscillation will be observable only on a single cell unless we synchronize the cell cycles of the cell on the plate. | ||
Revision as of 19:09, 2 August 2007
Project 1 Mexican Wave
FIRST VIEW
- Our first thought was to create a pattern by the change of colors in a concentration dependent manner; so that the production of a protein at the end of a different event would trigger the pattern formation, just like a goal triggering a *Mexican wave in soccer games.
- The plan was to have a plate having a lawn of same type E. coli cells having 3 constructs. Each construct would have a promoter activated by a different level of the initial signal protein and a reporter fluorescent protein coding part.
- The triggering protein A is being dropped on the middle of the plate, creating a concentration gradient on the plate as in the figure. Acording to this plan the color change will be once, and then by the rising level of [A], the plate will all become green.
SECOND VIEW
- The second view was to make this color change continious and independent of a created concentration gradient. So first idea came up to be coupling this oscillation to a already oscillating protein, maybe to cell cycle. But then this oscillation will be observable only on a single cell unless we synchronize the cell cycles of the cell on the plate.
