Bologna University/Plates preparation
From 2007.igem.org
(Difference between revisions)
Revision as of 11:03, 3 August 2007
- 1. Autoclave LB medium with 2% agar.
- 2. Cool at 50 °C to prevent agar polymerization.
- 3. Before preparing plates add antibiotic (Ampicillin 1000X or Kanamicin 200X).
- 4. Put about 20 ml of medium per plate.
- 5. Leave it solidify and store at 4°C.
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