Paris/August 3
From 2007.igem.org
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The double digestion allows us to insert the biobrick into biobrick plasmid.<br> | The double digestion allows us to insert the biobrick into biobrick plasmid.<br> | ||
The Pst1 digestion allows us to see if the mutagenesis worked (dgat gene not digested). | The Pst1 digestion allows us to see if the mutagenesis worked (dgat gene not digested). | ||
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| + | == TOPO cloning == | ||
| + | |||
| + | *P7 | ||
| + | *P7 | ||
| + | *P8 | ||
Revision as of 15:52, 3 August 2007
DGAT biobrick: digestion, cloning
- EcoR1-Pst1 digestion
The double digestion allows us to insert the biobrick into biobrick plasmid.
The Pst1 digestion allows us to see if the mutagenesis worked (dgat gene not digested).
TOPO cloning
- P7
- P7
- P8
