Paris/August 3
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== TOPO cloning == | == TOPO cloning == | ||
| - | + | {| | |
| - | + | |- style="background: #ccccff;" | |
| - | + | ! colspan="5" style="background: #ccccff;" | PCR products | |
| + | |- style="background: #ccccff; text-align: center;" | ||
| + | |width=5%| Number | ||
| + | |width=10%| Name | ||
| + | |width=15%| Oligo F | ||
| + | |width=15%| Oligo R | ||
| + | |width=10%| Matrix | ||
| + | |width=5%| Length | ||
| + | |width=25%| Comments | ||
| + | |width=5%| Product Left ? | ||
| + | |width=5%| Date | ||
| + | |[[Paris/July 17| July 17]], [[Paris/July 30| July 30]] | ||
| + | |- style="background: #cccccc;" | ||
| + | | style="background: #ccffcc;" |P6 | ||
| + | |Lox66-DapASubtilis | ||
| + | |O8 Lox66-DapASubt-F | ||
| + | |O9 DapASubt-R | ||
| + | |B.Subtilis 168 | ||
| + | |979 | ||
| + | | | ||
| + | | | ||
| + | |[[Paris/July 17| July 17]],[[Paris/July 27| July 27]],[[Paris/July 30| July 30]] | ||
| + | |- style="background: #cccccc;" | ||
| + | | style="background: #ccffcc;" |P7 | ||
| + | |Lox71-FtsZ | ||
| + | | O1 Lox71-FtsZ-F | ||
| + | | O2 FtsZ-R | ||
| + | |MG1655 | ||
| + | |1260 | ||
| + | |EcoRI site in FtsZ not mutated | ||
| + | | | ||
| + | |[[Paris/July 17| July 17]],[[Paris/July 30| July 30]] | ||
| + | |- style="background: #cccccc;" | ||
| + | | style="background: #ccffcc;" |P8 | ||
| + | |RBS-DapAColi | ||
| + | | O20 RBS-DapAColi-F | ||
| + | | O7 DapAColi-R | ||
| + | | MG1655 | ||
| + | |952 | ||
| + | |B0030-DapAColi | ||
| + | | | ||
| + | |} | ||
Revision as of 15:55, 3 August 2007
DGAT biobrick: digestion, cloning
- EcoR1-Pst1 digestion
The double digestion allows us to insert the biobrick into biobrick plasmid.
The Pst1 digestion allows us to see if the mutagenesis worked (dgat gene not digested).
TOPO cloning
| PCR products | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Number | Name | Oligo F | Oligo R | Matrix | Length | Comments | Product Left ? | Date | July 17, July 30 |
| P6 | Lox66-DapASubtilis | O8 Lox66-DapASubt-F | O9 DapASubt-R | B.Subtilis 168 | 979 | July 17, July 27, July 30 | |||
| P7 | Lox71-FtsZ | O1 Lox71-FtsZ-F | O2 FtsZ-R | MG1655 | 1260 | EcoRI site in FtsZ not mutated | July 17, July 30 | ||
| P8 | RBS-DapAColi | O20 RBS-DapAColi-F | O7 DapAColi-R | MG1655 | 952 | B0030-DapAColi | |||
