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Revision as of 15:55, 3 August 2007 (view source) Landrain (Talk | contribs) (→TOPO cloning) ← Older edit		Revision as of 15:56, 3 August 2007 (view source) Landrain (Talk | contribs) (→TOPO cloning) Newer edit →
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	|width=25%| Comments		|width=25%| Comments
	|width=5%| Product Left ?		|width=5%| Product Left ?
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	|[[Paris/July 17| July 17]], [[Paris/July 30| July 30]]		|[[Paris/July 17| July 17]], [[Paris/July 30| July 30]]
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-	|[[Paris/July 17| July 17]],[[Paris/July 27| July 27]],[[Paris/July 30| July 30]]
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	| style="background: #ccffcc;" |P7		| style="background: #ccffcc;" |P7
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	|EcoRI site in FtsZ not mutated		|EcoRI site in FtsZ not mutated
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-	|[[Paris/July 17| July 17]],[[Paris/July 30| July 30]]
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	| style="background: #ccffcc;" |P8		| style="background: #ccffcc;" |P8

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Revision as of 15:56, 3 August 2007

DGAT biobrick: digestion, cloning

• EcoR1-Pst1 digestion

The double digestion allows us to insert the biobrick into biobrick plasmid.
The Pst1 digestion allows us to see if the mutagenesis worked (dgat gene not digested).

TOPO cloning

Number	Name	Oligo F	Oligo R	Matrix	Length	Comments	Product Left ?	July 17, July 30
P6	Lox66-DapASubtilis	O8 Lox66-DapASubt-F	O9 DapASubt-R	B.Subtilis 168	979
P7	Lox71-FtsZ	O1 Lox71-FtsZ-F	O2 FtsZ-R	MG1655	1260	EcoRI site in FtsZ not mutated
P8	RBS-DapAColi	O20 RBS-DapAColi-F	O7 DapAColi-R	MG1655	952	B0030-DapAColi