Alberta/Calender/August
From 2007.igem.org
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| + | Justin, can you trade me for Wednesday, please???? I will owe you big. | ||
ED,VH,AF | ED,VH,AF | ||
Revision as of 16:59, 4 August 2007
August
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To July 2007
To September 2007
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August 1
Schedule: CZ,NG,VH
General Notes:
1. Run gel of B0034 SpeI/PstI double digest; extract gel with QIAgen kit; stored in freezer.
2. PCR for
a. Enny + B0034 miniprep 1 to 8
b. Betty colonies (10)
c. Buddy colonies (10)
Follow James' protocol. Also see blackboard for James' comment about PCR.
For tomorrow
1. run gel of PCR prodcut which are in the thermocycler �
2. check restreaked single colonies of the Betty and Buddy colony PCR
3. grow up Betty and Buddy overnight and for miniprep and ligation later
4. Make Amp plates (we only have 4 more)
5. Autoclave test tubes
Quotes of the day: We are tired.
August 2
Modeling Meeting @ 1830hrs in CAB373
General Meeting @ 1900hrs in CAB373
Party afterward's @ Garneau pub
Schedule:
MC, JP & AF are meeting at 1700hrs to get some stuff done before the meeting happens; come join in the fun if you'd like.
Lab Notes:
Made AMP plates
Autoclaved test tubes
Ran gel of ?
Started O/N of Buddy & Betty
Checked restreaked colonies of Buddy & Betty --> positive growth except for 9&10 but this is expected because they are controls
James did a loving PCR of Benny in B0034
-MC, ED, JP, JB
August 3
Schedule:
Nik cant make it he just isn't crazy enough looks like justin is currently the only one crazy enough
Michelle & Justin are now the long weekend crew - be prepared for ILLUSIONS!!!! or perhaps just some awesome pipetting techniques while they're prepping to champ out at the ULTIMATE pipetting Olympiads.
Meeting @ 1700hrs
Captain's Log, Stardate: 080307-1153
Minipreps of lifeforms Buddy and Betty
Not enough room in box with blue tape, designed a new holding vessel (i.e. box with white tape below box with blue tape in the freezer) for minipreps of aforementioned lifeforms.
-JB
Made gel
Digested (double) Buddy & Betty minipreps
Digest (single) I0500 - from tube we believe is a miniprep in the -20 freezer
weekend to-do list
-JP, ML, MC
August 4
Meeting at 1000hrs - Michelle will definately be there at 1000 Justin is expected at 1030
August 5
Meeting at 1000hrs - Michelle will definately be there at 1000 Justin is expected at 1030
August 6
Meeting at 1000hrs - Michelle will definately be there at 1000 Justin is expected at 1030
August 7
Schedule:
Justin, can you trade me for Wednesday, please???? I will owe you big.
ED,VH,AF
August 8
Schedule:
JP,NK,MC
August 9
Schedule:
ED,AF,JG
August 10
Schedule:
CZ, MC, JG
August 11
Schedule:
JP, AL, NK
August 12
Schedule:
CZ, AL, NK
August 13
August 14
August 15
August 16
August 17
August 18
August 19
August 20
August 21
August 22
August 23
August 24
August 25
August 26
August 27
August 28
August 29
August 30
August 31
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