Mechanism II
From 2007.igem.org
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[[Image:TJU_TEAM2_MIIFiguer4.jpg|thumb|600px|center|'''Figure 4 the riboflavin synthesis pathway''']] | [[Image:TJU_TEAM2_MIIFiguer4.jpg|thumb|600px|center|'''Figure 4 the riboflavin synthesis pathway''']] | ||
We propose to change the prmoters related to riboflavin synthesis of Yeast to strong promter ADH1. The two-step-gene replacement method is used in our project. Take RIB1 as an example. Take the RIB1gene as three regions: region1, region2 and region2 (figure5) | We propose to change the prmoters related to riboflavin synthesis of Yeast to strong promter ADH1. The two-step-gene replacement method is used in our project. Take RIB1 as an example. Take the RIB1gene as three regions: region1, region2 and region2 (figure5) | ||
| + | [[Image:TJU_TEAM2_MIIFiguer5.jpg|thumb|600px|center|'''Figure 5 the regions in RIB1 gene''']] | ||
Revision as of 10:06, 5 August 2007
Yeast can produce riboflavin itself, but it can only supply its own metabolism without accumulation. There are 6 genes related to the riboflavin production. The patyway of riboflavin synthesis is showed below:
We propose to change the prmoters related to riboflavin synthesis of Yeast to strong promter ADH1. The two-step-gene replacement method is used in our project. Take RIB1 as an example. Take the RIB1gene as three regions: region1, region2 and region2 (figure5)
