NYMU Taipei/Lab Notes/2007 8 6&7

From 2007.igem.org

(Difference between revisions)
Line 3: Line 3:
** primer design for [http://www.informatics.jax.org/searches/accession_report.cgi?id=MGI%3A96573 insulin II of Mus musculus]
** primer design for [http://www.informatics.jax.org/searches/accession_report.cgi?id=MGI%3A96573 insulin II of Mus musculus]
*** primer for A chain
*** primer for A chain
 +
***left: <font color="red">ATTGTAGATCAGTGCTGCAC</font>
 +
***right: <font color="red">GTTGCAGTAGTTCTCCAGCT</font>
*** primer for B chain
*** primer for B chain
**** left: GTTTCTTCGAATTCGCGGCCGCTTCTAGATG<font color="red">TTTGTCAAGCAGCACCTTTG</font>
**** left: GTTTCTTCGAATTCGCGGCCGCTTCTAGATG<font color="red">TTTGTCAAGCAGCACCTTTG</font>
**** right: GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTA<font color="red">GGACATGGGTGTGTAGAAGA</font>
**** right: GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTA<font color="red">GGACATGGGTGTGTAGAAGA</font>
* purchase of BCRC human insulin clone (ordered not paid)
* purchase of BCRC human insulin clone (ordered not paid)

Revision as of 14:03, 6 August 2007

Lab schedule

  • mouse insulin gene cloning (delay to 8/7)
    • primer design for [http://www.informatics.jax.org/searches/accession_report.cgi?id=MGI%3A96573 insulin II of Mus musculus]
      • primer for A chain
      • left: ATTGTAGATCAGTGCTGCAC
      • right: GTTGCAGTAGTTCTCCAGCT
      • primer for B chain
        • left: GTTTCTTCGAATTCGCGGCCGCTTCTAGATGTTTGTCAAGCAGCACCTTTG
        • right: GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAGGACATGGGTGTGTAGAAGA
  • purchase of BCRC human insulin clone (ordered not paid)
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