NYMU Taipei/Lab Notes/2007 8 6&7

From 2007.igem.org

(Difference between revisions)
Line 2: Line 2:
* mouse insulin gene cloning (delay to 8/7)
* mouse insulin gene cloning (delay to 8/7)
** primer design for [http://www.informatics.jax.org/searches/accession_report.cgi?id=MGI%3A96573 insulin II of Mus musculus]
** primer design for [http://www.informatics.jax.org/searches/accession_report.cgi?id=MGI%3A96573 insulin II of Mus musculus]
-
*** primer for A chain
+
*** primer for A chain (90-110: GIVDQCCTSICSLYQLENYCN)
**** left: GTTTCTTCGAATTCGCGGCCGCTTCTAGATG<font color="red">ATTGTAGATCAGTGCTGCAC</font>
**** left: GTTTCTTCGAATTCGCGGCCGCTTCTAGATG<font color="red">ATTGTAGATCAGTGCTGCAC</font>
**** right: GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTA<font color="red">GTTGCAGTAGTTCTCCAGCT</font>
**** right: GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTA<font color="red">GTTGCAGTAGTTCTCCAGCT</font>

Revision as of 14:09, 6 August 2007

Lab schedule

  • mouse insulin gene cloning (delay to 8/7)
    • primer design for [http://www.informatics.jax.org/searches/accession_report.cgi?id=MGI%3A96573 insulin II of Mus musculus]
      • primer for A chain (90-110: GIVDQCCTSICSLYQLENYCN)
        • left: GTTTCTTCGAATTCGCGGCCGCTTCTAGATGATTGTAGATCAGTGCTGCAC
        • right: GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAGTTGCAGTAGTTCTCCAGCT
      • primer for B chain
        • left: GTTTCTTCGAATTCGCGGCCGCTTCTAGATGTTTGTCAAGCAGCACCTTTG
        • right: GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAGGACATGGGTGTGTAGAAGA
  • purchase of BCRC human insulin clone (ordered not paid)
  • antibiotics LB plate preparation
    • ampicillin
    • kanamycin
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