BerkiGEM2007-KristinConstructionFile28
From 2007.igem.org
(Difference between revisions)
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<pre> | <pre> | ||
| - | Composite part of T7 promoter + hemA from R. capsulatus | + | Composite part of T7 promoter + library hemA from R. capsulatus |
Digest pBca9145-I716105 (EcoR1/BamHI, 2063+44, L) | Digest pBca9145-I716105 (EcoR1/BamHI, 2063+44, L) | ||
Sub into pBca9145-I716156 (EcoR1/Bg1II, 3313+1027, L) | Sub into pBca9145-I716156 (EcoR1/Bg1II, 3313+1027, L) | ||
Product is pBca9145-I716178 | Product is pBca9145-I716178 | ||
</pre> | </pre> | ||
Latest revision as of 18:08, 8 August 2007
Composite part of T7 promoter + library hemA from R. capsulatus Digest pBca9145-I716105 (EcoR1/BamHI, 2063+44, L) Sub into pBca9145-I716156 (EcoR1/Bg1II, 3313+1027, L) Product is pBca9145-I716178
