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Revision as of 20:25, 8 August 2007 (view source) Vaibhaviu (Talk | contribs) ← Older edit		Latest revision as of 20:25, 8 August 2007 (view source) Vaibhaviu (Talk | contribs)
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	PCR ca1099F/G00101 on Part A (pBca9145-Bca1090) (553 bp)		PCR ca1099F/G00101 on Part A (pBca9145-Bca1090) (553 bp)
-	Digest pBca9145-Bca1090 (BsaI/BamHI, 989+1102, S)	+	Digest pBca9145-Bca1090 (BsaI/BamHI, 989+1102, S)
	Product is I716258		Product is I716258
	Ligate with Part B		Ligate with Part B
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	PCR ca998/ca1099R on Part B (pBca9145-I716253) (3393 bp)		PCR ca998/ca1099R on Part B (pBca9145-I716253) (3393 bp)
-	Digest pBca9145-I716253 (BsaI/BglII, 2512 + 777, L)	+	Digest pBca9145-I716253 (BsaI/BglII, 2512 + 777, L)
	Product is I716259		Product is I716259
	Ligate with Part A		Ligate with Part A

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Latest revision as of 20:25, 8 August 2007

Method Used: dBBS

PCR ca1099F/G00101 on Part A (pBca9145-Bca1090)	(553 bp)
Digest pBca9145-Bca1090				(BsaI/BamHI, 989+1102, S)
Product is I716258
Ligate with Part B
-------------------------------------
ca1099F – ccaaaAGATCTatgaaacgttttagtctggc
G00101 – gctagCTCGAGttaGGATCCttacttaattacaccacaggc


PCR ca998/ca1099R on Part B (pBca9145-I716253)	(3393 bp)
Digest pBca9145-I716253				(BsaI/BglII, 2512 + 777, L)
Product is I716259
Ligate with Part A
-------------------------------------
ca998 – gtatcacgaggcagaatttcag
ca1099R – gctgataaatctggagccgg

Final product is I716260